Electrospun fibrinogen-PLA nanofibres for vascular tissue engineering

Gugutkov, D., Gustavsson, J., Cantini, M. , Salmeron-Sanchez, M. and Altankov, G. (2017) Electrospun fibrinogen-PLA nanofibres for vascular tissue engineering. Journal of Tissue Engineering and Regenerative Medicine, 11(10), pp. 2774-2784. (doi:10.1002/term.2172) (PMID:27238477)

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Abstract

Here we report on the development of a new type of hybrid fibrinogen–polylactic acid (FBG–PLA) nanofibres (NFs) with improved stiffness, combining the good mechanical properties of PLA with the excellent cell recognition properties of native FBG. We were particularly interested in the dorsal and ventral cell response to the nanofibres' organization (random or aligned), using human umbilical endothelial cells (HUVECs) as a model system. Upon ventral contact with random NFs, the cells developed a stellate-like morphology with multiple projections. The well-developed focal adhesion complexes suggested a successful cellular interaction. However, time-lapse analysis shows significantly lowered cell movements, resulting in the cells traversing a relatively short distance in multiple directions. Conversely, an elongated cell shape and significantly increased cell mobility were observed in aligned NFs. To follow the dorsal cell response, artificial wounds were created on confluent cell layers previously grown on glass slides and covered with either random or aligned NFs. Time-lapse analysis showed significantly faster wound coverage (within 12 h) of HUVECs on aligned samples vs. almost absent directional migration on random ones. However, nitric oxide (NO) release shows that endothelial cells possess lowered functionality on aligned NFs compared to random ones, where significantly higher NO production was found. Collectively, our studies show that randomly organized NFs could support the endothelization of implants while aligned NFs would rather direct cell locomotion for guided neovascularization.

Item Type:Articles
Additional Information:Funded by: BIOSURFACES, Intramoral program CIBER–BBN (Spain) and the European Commission (EC) FP7-People program Industry–Academia Partnerships and Pathways (IAPP). Grant Number: 324386 FIBROGELNET Spanish Ministry of Science and Innovation. Grant Number: MAT2012-38359-C03-03 HEALINSYNERGY
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel and Cantini, Dr Marco
Authors: Gugutkov, D., Gustavsson, J., Cantini, M., Salmeron-Sanchez, M., and Altankov, G.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Journal of Tissue Engineering and Regenerative Medicine
Publisher:John Wiley and Sons Ltd
ISSN:1932-6254
ISSN (Online):1932-7005
Published Online:30 May 2016
Copyright Holders:Copyright © 2016 John Wiley and Sons, Ltd
First Published:First published in Journal of Tissue Engineering and Regenerative Medicine 2016
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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