Stable isotope techniques for the assessment of host and microbiota response during gastrointestinal dysfunction

Butler, R. N., Kosek, M., Krebs, N. F., Loechl, C., Loy, A., Owino, V., Zimmermann, M. B. and Morrison, D. J. (2017) Stable isotope techniques for the assessment of host and microbiota response during gastrointestinal dysfunction. Journal of Pediatric Gastroenterology and Nutrition, 64(1), pp. 8-14. (doi: 10.1097/MPG.0000000000001373) (PMID:27632432)

Abstract

The International Atomic Energy Agency convened a technical meeting on environmental enteric dysfunction (EED) in Vienna (28th-30th October 2015; https://nucleus.iaea.org/HHW/Nutrition/EED_Technical_Meeting/index.html) to bring together international experts in the fields of EED, nutrition and stable isotope technologies. Advances in stable isotope labelling techniques open up new possibilities to improve our understanding of gastrointestinal dysfunction and the role of the microbiota in host health. In the context of EED, little is known about the role gut dysfunction may play in macro- and micronutrient bioavailability and requirements and what the consequences may be for nutritional status and linear growth. Stable isotope labelling techniques have been used to assess intestinal mucosal injury and barrier function, carbohydrate digestion and fermentation, protein derived amino acid bioavailability and requirements, micronutrient bioavailability and to track microbe-microbe and microbe-host interactions at the single cell level. The non-invasive nature of stable isotope technologies potentially allows for low-hazard, field deployable tests of gut dysfunction that are applicable across all age-groups. The purpose of this review is to assess the state-of-the-art in the use of stable isotope technologies and to provide a perspective on where these technologies can be exploited to further our understanding of gut dysfunction in EED.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Morrison, Professor Douglas
• Authors: Butler, R. N., Kosek, M., Krebs, N. F., Loechl, C., Loy, A., Owino, V., Zimmermann, M. B., and Morrison, D. J.
• College/School: College of Science and Engineering > Scottish Universities Environmental Research Centre
• Journal Name: Journal of Pediatric Gastroenterology and Nutrition
• Publisher: Lippincott, Williams & Wilkins
• ISSN: 0277-2116
• ISSN (Online): 1536-4801
• Published Online: 14 April 2016
• Copyright Holders: Copyright © 2016 European Society for Pediatric Gastroenterology, Hepatology, and Nutrition and North American Society for Pediatric Gastroenterology, Hepatology, and Nutrition
• First Published: First published in Journal of Pediatric Gastroenterology and Nutrition 64(1): 8-14
• Publisher Policy: Reproduced under a Creative Commons License