Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 3. Untargeted metabolomics

Thomas, F., Mudaliar, M. , Tassi, R., Mcneilly, T., Burchmore, R., Burgess, K., Herzyk, P., Zadoks, R. and Eckersall, P. (2016) Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 3. Untargeted metabolomics. Molecular BioSystems, 12(9), pp. 2762-2769. (doi:10.1039/C6MB00289G) (PMID:27412568)

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Abstract

Intramammary infection leading to bovine mastitis is the leading disease problem affecting dairy cows and has marked effects on the milk produced by infected udder quarters. An experimental model of Streptococcus uberis mastitis has previously been investigated for clinical, immunological and pathophysiological alteration in milk, and has been the subject of peptidomic and quantitative proteomic investigation. The same sample set has now been investigated with a metabolomics approach using liquid chromatography and mass spectrometry. The analysis revealed over 3000 chromatographic peaks, of which 690 were putatively annotated with a metabolite. Hierarchical clustering analysis and principal component analysis demonstrated that metabolite changes due to S. uberis infection were maximal at 81 hours post challenge with metabolites in the milk from the resolution phase at 312 hours post challenge being closest to the pre-challenge samples. Metabolic pathway analysis revealed that the majority of the metabolites mapped to carbohydrate and nucleotide metabolism show a decreasing trend in concentration up to 81 hours post-challenge whereas an increasing trend was found in lipid metabolites and di-, tri- and tetra-peptides up to the same time point. The increase in these peptides coincides with an increase in larger peptides found in the previous peptidomic analysis and is likely to be due to protease degradation of milk proteins. Components of bile acid metabolism, linked to the FXR pathway regulating inflammation, were also increased. Metabolomic analysis of the response in milk during mastitis provides an essential component to the full understanding of the mammary gland’s response to infection.

Item Type:Articles
Keywords:Metabolomics, mastitis, Streptococcus uberis.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Eckersall, Professor Peter and Zadoks, Professor Ruth and Burchmore, Dr Richard and Mudaliar, Dr Manikhandan and Herzyk, Dr Pawel and Burgess, Dr Karl and Mcneilly, Dr Tom
Authors: Thomas, F., Mudaliar, M., Tassi, R., Mcneilly, T., Burchmore, R., Burgess, K., Herzyk, P., Zadoks, R., and Eckersall, P.
Subjects:S Agriculture > SF Animal culture > SF600 Veterinary Medicine
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Molecular BioSystems
Publisher:Royal Society of Chemistry
ISSN:1742-206X
ISSN (Online):1742-2051
Published Online:14 July 2016
Copyright Holders:Copyright © 2016 The Royal Society of Chemistry
First Published:First published in Molecular BioSystems 12(9): 2762-2769
Publisher Policy:Reproduced under a Creative Commons License
Data DOI:10.1039/c6mb00289g

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
594271Integrated Health - Polyomics and Systems Biomedicine (ISSF Bid)Anna DominiczakWellcome Trust (WELLCOME)097821/Z/11/ZRI CARDIOVASCULAR & MEDICAL SCIENCES