Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 1. High abundance proteins, acute phase proteins and peptidomics

Thomas, F., Mullen, W. , Tassi, R., Ramírez-Torres, A., Mudaliar, M. , Mcneilly, T., Zadoks, R., Burchmore, R. and Eckersall, P. (2016) Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 1. High abundance proteins, acute phase proteins and peptidomics. Molecular BioSystems, 12(9), pp. 2735-2747. (doi:10.1039/C6MB00239K) (PMID:27412456) (PMCID:PMC5048397)

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Abstract

A peptidomic investigation of milk from an experimental model of Streptococcus uberis mastitis in dairy cows has incorporated a study of milk high abundance and acute phase (APP) proteins as well as analysis of low molecular weight peptide biomarkers. Intramammary infection (IMI) with S. uberis caused a shift in abundance from caseins, β-lactoglobulin and α-lactalbumin to albumin, lactoferrin and IgG with the increase in lactoferrin occurring last. The APP response of haptoglobin, mammary associated serum amyloid A3 and C-reactive protein occurred between 30–48 hours post challenge with peak concentrations of APPs at 72–96 hours post challenge and declined thereafter at a rate resembling the fall in bacterial count rather than the somatic cell count. A peptide biomarker panel for IMI based on capillary electrophoresis and mass spectrometry was developed. It comprised 77 identified peptides (IMI77) composed mainly of casein derived peptides but also including peptides of glycosylation dependent cell adhesion molecule and serum amyloid A. The panel had a biomarker classification score that increased from 36 hour to 81 hour post challenge, significantly differentiating infected from non-infected milk, thus suggesting potential as a peptide biomarker panel of bovine mastitis and specifically that of S. uberis origin. The use of omic technology has shown a multifactorial cross system reaction in high and low abundance proteins and their peptide derivatives with changes of over a thousand fold in analyte levels in response to S. uberis infection.

Item Type:Articles
Keywords:Peptidomics, Mastitis, Streptococcus uberis
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Mullen, Dr William and Burchmore, Dr Richard and Eckersall, Professor Peter and Mcneilly, Dr Tom and Zadoks, Professor Ruth and Mudaliar, Dr Manikhandan
Authors: Thomas, F., Mullen, W., Tassi, R., Ramírez-Torres, A., Mudaliar, M., Mcneilly, T., Zadoks, R., Burchmore, R., and Eckersall, P.
Subjects:S Agriculture > SF Animal culture > SF600 Veterinary Medicine
College/School:College of Medical Veterinary and Life Sciences
College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Molecular BioSystems
Publisher:Royal Society of Chemistry
ISSN:1742-206X
ISSN (Online):1742-2051
Published Online:14 July 2016
Copyright Holders:Copyright © 2016 The Royal Society of Chemistry
First Published:First published in Molecular BioSystems 12(9): 2735-2747
Publisher Policy:Reproduced under a Creative Commons License

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