Paper-origami-based multiplexed malaria diagnostics from whole blood

Xu, G., Nolder, D., Reboud, J. , Oguike, M. C., van Schalkwyk, D. A., Sutherland, C. J. and Cooper, J. M. (2016) Paper-origami-based multiplexed malaria diagnostics from whole blood. Angewandte Chemie (International Edition), 55(49), pp. 15250-15253. (doi:10.1002/anie.201606060) (PMID:27554333) (PMCID:PMC5132111)

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Abstract

We demonstrate, for the first time, the multiplexed determination of microbial species from whole blood using the paper-folding technique of origami to enable the sequential steps of DNA extraction, loop-mediated isothermal amplification (LAMP), and array-based fluorescence detection. A low-cost handheld flashlight reveals the presence of the final DNA amplicon to the naked eye, providing a “sample-to-answer” diagnosis from a finger-prick volume of human blood, within 45 min, with minimal user intervention. To demonstrate the method, we showed the identification of three species of Plasmodium, analyzing 80 patient samples benchmarked against the gold-standard polymerase chain reaction (PCR) assay in an operator-blinded study. We also show that the test retains its diagnostic accuracy when using stored or fixed reference samples.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cooper, Professor Jonathan and Reboud, Dr Julien and Xu, Mr Gaolian
Authors: Xu, G., Nolder, D., Reboud, J., Oguike, M. C., van Schalkwyk, D. A., Sutherland, C. J., and Cooper, J. M.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Angewandte Chemie (International Edition)
Publisher:Wiley - VCH Verlag GmbH & Co. KGaA
ISSN:1433-7851
ISSN (Online):1521-3773
Published Online:24 August 2016
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Angewandte Chemie (International Edition) 55(49):15250-15253
Publisher Policy:Reproduced under a Creative Commons License
Data DOI:10.5525/gla.researchdata.339

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
553521Next Generation Analytical Tools: Application to Protein Oxidations that affect Human Health and WellbeingJonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/I017887/1ENG - BIOMEDICAL ENGINEERING
617021Advanced Diagnostics using PhononicsJonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/K027611/1ENG - BIOMEDICAL ENGINEERING