Paper-origami-based multiplexed malaria diagnostics from whole blood

Xu, G., Nolder, D., Reboud, J. , Oguike, M. C., van Schalkwyk, D. A., Sutherland, C. J. and Cooper, J. M. (2016) Paper-origami-based multiplexed malaria diagnostics from whole blood. Angewandte Chemie (International Edition), 55(49), pp. 15250-15253. (doi: 10.1002/anie.201606060) (PMID:27554333) (PMCID:PMC5132111)

Abstract

We demonstrate, for the first time, the multiplexed determination of microbial species from whole blood using the paper-folding technique of origami to enable the sequential steps of DNA extraction, loop-mediated isothermal amplification (LAMP), and array-based fluorescence detection. A low-cost handheld flashlight reveals the presence of the final DNA amplicon to the naked eye, providing a “sample-to-answer” diagnosis from a finger-prick volume of human blood, within 45 min, with minimal user intervention. To demonstrate the method, we showed the identification of three species of Plasmodium, analyzing 80 patient samples benchmarked against the gold-standard polymerase chain reaction (PCR) assay in an operator-blinded study. We also show that the test retains its diagnostic accuracy when using stored or fixed reference samples.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Cooper, Professor Jonathan and Reboud, Dr Julien and Xu, Mr Gaolian
• Authors: Xu, G., Nolder, D., Reboud, J., Oguike, M. C., van Schalkwyk, D. A., Sutherland, C. J., and Cooper, J. M.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: Angewandte Chemie (International Edition)
• Publisher: Wiley - VCH Verlag GmbH & Co. KGaA
• ISSN: 1433-7851
• ISSN (Online): 1521-3773
• Published Online: 24 August 2016
• Copyright Holders: Copyright © 2016 The Authors
• First Published: First published in Angewandte Chemie (International Edition) 55(49):15250-15253
• Publisher Policy: Reproduced under a Creative Commons License
• Data DOI: 10.5525/gla.researchdata.339

Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
55352	1	Next Generation Analytical Tools: Application to Protein Oxidations that affect Human Health and Wellbeing	Jonathan Cooper	Engineering & Physical Sciences Research Council (EPSRC)	EP/I017887/1	ENG - BIOMEDICAL ENGINEERING
61702	1	Advanced Diagnostics using Phononics	Jonathan Cooper	Engineering & Physical Sciences Research Council (EPSRC)	EP/K027611/1	ENG - BIOMEDICAL ENGINEERING