Structure and behaviour of proteins, nucleic acids and viruses from vibrational Raman optical activity

Barron, L.D., Blanch, E.W., McColl, I.H., Syme, C.D. , Hecht, L. and Nielsen, K. (2003) Structure and behaviour of proteins, nucleic acids and viruses from vibrational Raman optical activity. Spectroscopy, 17(2-3), pp. 101-126. (doi: 10.1155/2003/787940)

Abstract

On account of its sensitivity to chirality Raman optical activity (ROA), which may be measured as a small difference in vibrational Raman scattering from chiral molecules in right- and left-circularly polarized incident light, is a powerful probe of structure and behaviour of biomolecules in aqueous solution. Protein ROA spectra provide information on the secondary and tertiary structure of the polypeptide backbone, hydration, side chain conformation and structural elements present in denatured states. Nucleic acid ROA spectra provide information on the sugar ring conformation, the base stacking arrangement and the mutual orientation of the sugar and base rings around the C-N glycosidic link. The ROA spectra of intact viruses provide information on the folds of the coat proteins and the nucleic acid structure. The large number of structure-sensitive bands in protein ROA spectra is especially favourable for fold determination using pattern recognition techniques. This article gives a brief account of the ROA technique and presents the ROA spectra of a selection of proteins, nucleic acids and viruses that illustrate the applications of ROA spectroscopy in biomolecular research.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Syme, Dr Christopher and Hecht, Dr Lutz and Barron, Professor Laurence
• Authors: Barron, L.D., Blanch, E.W., McColl, I.H., Syme, C.D., Hecht, L., and Nielsen, K.
• College/School: College of Science and Engineering > School of Chemistry
• Journal Name: Spectroscopy
• Publisher: I O S Press
• ISSN: 0712-4813
• ISSN (Online): 1875-922X
• Published Online: 01 January 2003

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Project Code	Award No	Project Name	Principal Investigator	Funder's Name	Funder Ref	Lead Dept
39851	1	Development of an ultraviolet Raman optical activity instrument for pre-resonant protein and virus studies	Lutz Hecht	Engineering & Physical Sciences Research Council (EPSRC)	EP/C535154/1	Chemistry