Protein adsorption as a key mediator in the nanotopographical control of cell behavior

Mpoyi, E. N., Cantini, M. , Reynolds, P. M., Gadegaard, N., Dalby, M. J. and Salmerón-Sánchez, M. (2016) Protein adsorption as a key mediator in the nanotopographical control of cell behavior. ACS Nano, 10(7), pp. 6638-6647. (doi:10.1021/acsnano.6b01649) (PMID:27391047) (PMCID:PMC4980054)

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Abstract

Surface nanotopography is widely employed to control cell behavior and in particular controlled disorder has been shown to be important in cell differentiation/maturation. However, extracellular matrix proteins, such as fibronectin (FN), initially adsorbed on a biomaterial surface are known to mediate the interaction of synthetic materials with cells. In this work, we examine the effect of nanotopography on cell behavior through this adsorbed layer of adhesive proteins using a nanostructured polycarbonate surface comprising 150 nm-diameter pits originally defined using electron beam lithography. We address the effect of this nanopitted surface on FN adsorption and subsequently on cell morphology and behavior using C2C12 myoblasts. Wettability measurements and atomic force microscopy imaging showed that protein is adsorbed both within the inter-pits spaces and inside the nanopits. Cells responded to this coated nanotopography with the formation of fewer but larger focal adhesions and by mimicking the pit patterns within their cytoskeleton, nanoimprinting, ultimately achieving higher levels of myogenic differentiation compared to a flat control. Both focal adhesion assembly and nanoimprinting were found to be dependent on cell contractility and are adversely affected by the use of blebbistatin. Our results demonstrate the central role of the nanoscale protein interface in mediating cell-nanotopographical interactions and implicate this interface as helping control the mechanotransductive cascade.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel and Gadegaard, Professor Nikolaj and Cantini, Dr Marco and Dalby, Professor Matthew and Reynolds, Mr Paul
Authors: Mpoyi, E. N., Cantini, M., Reynolds, P. M., Gadegaard, N., Dalby, M. J., and Salmerón-Sánchez, M.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:ACS Nano
Publisher:American Chemical Society
ISSN:1936-0851
ISSN (Online):1936-086X
Published Online:08 July 2016
Copyright Holders:Copyright © 2016 American Chemical Society
First Published:First published in ACS Nano 10(7):6638-6647
Publisher Policy:Reproduced under a Creative Commons License
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
653731Synergistic microenvironments for non-union bone defectsMatthew DalbyMedical Research Council (MRC)MR/L022710/1RI MOLECULAR CELL & SYSTEMS BIOLOGY
470563DTC in cell and proteomic technologies (continuation)Jonathan CooperEngineering & Physical Sciences Research Council (EPSRC)EP/F500424/1ENG - BIOMEDICAL ENGINEERING