McCaughey, L. C., Ritchie, N. D., Douce, G. R. , Evans, T. J. and Walker, D. (2016) Efficacy of species-specific protein antibiotics in a murine model of acute Pseudomonas aeruginosa lung infection. Scientific Reports, 6, 30201. (doi: 10.1038/srep30201) (PMID:27444885) (PMCID:PMC4957109)
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Abstract
Protein antibiotics, known as bacteriocins, are widely produced by bacteria for intraspecies competition. The potency and targeted action of bacteriocins suggests that they could be developed into clinically useful antibiotics against highly drug resistant Gram-negative pathogens for which there are few therapeutic options. Here we show that Pseudomonas aeruginosa specific bacteriocins, known as pyocins, show strong efficacy in a murine model of P. aeruginosa lung infection, with the concentration of pyocin S5 required to afford protection from a lethal infection at least 100-fold lower than the most commonly used inhaled antibiotic tobramycin. Additionally, pyocins are stable in the lung, poorly immunogenic at high concentrations and efficacy is maintained in the presence of pyocin specific antibodies after repeated pyocin administration. Bacteriocin encoding genes are frequently found in microbial genomes and could therefore offer a ready supply of highly targeted and potent antibiotics active against problematic Gram-negative pathogens.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Walker, Professor Daniel and Evans, Professor Tom and Ritchie, Dr Neil and MCCAUGHEY, Dr LAURA and Douce, Dr Gillian |
| Authors: | McCaughey, L. C., Ritchie, N. D., Douce, G. R., Evans, T. J., and Walker, D. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Scientific Reports |
| Publisher: | Nature Research |
| ISSN: | 2045-2322 |
| ISSN (Online): | 2045-2322 |
| Published Online: | 21 July 2016 |
| Copyright Holders: | Copyright © 2016 The Authors |
| First Published: | First published in Scientific Reports 6:30201 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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