Applicator for in-vitro ultrasound-activated targeted drug delivery

Gerold, B. et al. (2012) Applicator for in-vitro ultrasound-activated targeted drug delivery. In: 11th International Symposium on Therapeutic Ultrasound. 2012 AIP Conference Proceedings, New York, NY, 11–13 April 2011, pp. 100-105. (doi:10.1063/1.4757318)

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Abstract

Reducing toxicity and improving uptake of cancer drugs in tumors are important goals of targeted drug delivery (TDD). Ultrasonic drug release from various encapsulants has been a focus of many research groups. However, a single standard ultrasonic device, viable for use by biologists, is not currently present in the market. The device reported here is designed to allow investigation of the impact of ultrasound on cellular uptake and cell viability in-vitro. In it, single-element transducers with different operating frequencies are mounted below a standard 96-well plate. The plate is moved above the transducers, such that each line of wells can be sonicated at a different frequency. To assess the device, 96-well plates were seeded with cells and sonicated using different ultrasonic parameters, with and without doxorubicin. Cell viability was measured by colorimetric MTT assay and the uptake of doxorubicin by cells was also determined. The device proved to be highly viable in preliminary tests; it demonstrated that change in ultrasonic parameters produces different effect on cells. For example, increase in uptake of doxorubicin was demonstrated following ultrasound application. The growing interest in ultrasound-activated TDD emphasizes the need for standardization of the ultrasound device and the one reported here may offer some indications of how that may be achieved. It is planned to further improve the prototype by increasing the number of ultrasonic frequencies and degrees of freedom for each transducer.

Item Type:Conference Proceedings
Additional Information:This work is supported by the European Community’s Seventh Framework Program (FP7/2007-2013) under Grant Agreement no. 230674
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Gerold, Dr Bjoern and Cochran, Professor Alexander and Prentice, Dr Paul
Authors: Gerold, B., Gourevich, D., Volovick, A., Xu, D., Arditti, F., Prentice, P., Cochran, S., Gnaim, J., Medan, Y., Wang, L., and Melzer, A.
College/School:College of Science and Engineering > School of Engineering > Systems Power and Energy
ISSN:0094-243X
Published Online:03 October 2012

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