Low pH-induced cytopathic effect—a survey of seven hantavirus strains

McCaughey, C., Shi, X. , Elliott, R. M., Wyatt, D. E., O’Neill, H. J. and Coyle, P. V. (1999) Low pH-induced cytopathic effect—a survey of seven hantavirus strains. Journal of Virological Methods, 81(1-2), pp. 193-197. (doi:10.1016/S0166-0934(99)00042-7) (PMID:10488778)

Full text not currently available from Enlighten.

Abstract

Hantaviruses do not produce cytopathic effects (CPE) in cell culture. However, a syncytial CPE can be induced in 7-day cultures of hantavirus growing in Vero E6 cells by reduction of the pH to approximately 6.2 using a HEPES based buffer. The appearance of this acid induced CPE was examined for seven different hantavirus strains. The differences noted were striking and reflected the taxonomic differences between hantaviruses. At 10–100 TCID50% the size of syncytial foci was very large for Seoul type viruses and smallest for Puumala viruses. The size of syncytia for Hantaan (HTN) virus was intermediate between Puumala (PUU) and Seoul (SEO) type viruses.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Elliott, Professor Richard and Shi, Dr Xiaohong
Authors: McCaughey, C., Shi, X., Elliott, R. M., Wyatt, D. E., O’Neill, H. J., and Coyle, P. V.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Virological Methods
Publisher:Elsevier
ISSN:0166-0934
ISSN (Online):1879-0984
Published Online:14 October 1999

University Staff: Request a correction | Enlighten Editors: Update this record