IL-22 exacerbates weight loss in a murine model of chronic pulmonary Pseudomonas aeruginosa infection

Bayes, H. K., Ritchie, N. E., Ward, C., Corris, P. A., Brodlie, M. and Evans, T. (2016) IL-22 exacerbates weight loss in a murine model of chronic pulmonary Pseudomonas aeruginosa infection. Journal of Cystic Fibrosis, 16(6), pp. 759-768. (doi:10.1016/j.jcf.2016.06.008) (PMID:27375092) (PMCID:PMC5154339)

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Abstract

Background: Interleukin (IL)-22 is a critical mediator of mucosal immunity and tissue regeneration, protecting against a number of respiratory pathogens. Whether IL-22 confers protection against chronic Pseudomonas aeruginosa (PA) infection in cystic fibrosis (CF) is unknown. Methods: Explanted CF lungs were examined for IL-22 production and immune-localization. A murine model of persistent pulmonary PA infection was used to examine production of IL-22 following infective challenge. The role of IL-22 was examined using IL-22 knockout (KO) animals. Results: IL-22 is produced within the adult CF lung and localizes to the airway epithelium. IL-22 is produced by murine pulmonary lymph node cells following lung infection. The absence of IL-22 resulted in no significant difference in acute mortality, bacterial burden, chronic infection rates, histological changes or neutrophilic inflammation in the chronic PA infection model. However, IL-22 KO animals lost less weight following infection. Conclusion: IL-22 is produced in the CF lung and in response to PA infection yet is dispensable in protection against chronic pulmonary P. aeruginosa infection in a murine model. However, we identified a novel role for the cytokine in promoting infection-related weight-loss, a significant prognostic factor in the CF population.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Evans, Professor Tom and Ward, Dr Christopher and Ritchie, Dr Neil and Bayes, Dr Hannah
Authors: Bayes, H. K., Ritchie, N. E., Ward, C., Corris, P. A., Brodlie, M., and Evans, T.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Cystic Fibrosis
Publisher:Elsevier
ISSN:1569-1993
ISSN (Online):1873-5010
Published Online:01 July 2016
Copyright Holders:Copyright © 2016 The Authors
First Published:First published Journal of Cystic Fibrosis 2016
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
558681The Proinflammatory Th17 Response as a Therapeutic Target in Cystic Fibrosis Lung DiseaseTom EvansWellcome Trust (WELLCOME)094779/Z/10/ZIII - BACTERIOLOGY