Bayes, H. K., Ritchie, N. E., Ward, C., Corris, P. A., Brodlie, M. and Evans, T. (2016) IL-22 exacerbates weight loss in a murine model of chronic pulmonary Pseudomonas aeruginosa infection. Journal of Cystic Fibrosis, 16(6), pp. 759-768. (doi: 10.1016/j.jcf.2016.06.008) (PMID:27375092) (PMCID:PMC5154339)
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Abstract
Background: Interleukin (IL)-22 is a critical mediator of mucosal immunity and tissue regeneration, protecting against a number of respiratory pathogens. Whether IL-22 confers protection against chronic Pseudomonas aeruginosa (PA) infection in cystic fibrosis (CF) is unknown. Methods: Explanted CF lungs were examined for IL-22 production and immune-localization. A murine model of persistent pulmonary PA infection was used to examine production of IL-22 following infective challenge. The role of IL-22 was examined using IL-22 knockout (KO) animals. Results: IL-22 is produced within the adult CF lung and localizes to the airway epithelium. IL-22 is produced by murine pulmonary lymph node cells following lung infection. The absence of IL-22 resulted in no significant difference in acute mortality, bacterial burden, chronic infection rates, histological changes or neutrophilic inflammation in the chronic PA infection model. However, IL-22 KO animals lost less weight following infection. Conclusion: IL-22 is produced in the CF lung and in response to PA infection yet is dispensable in protection against chronic pulmonary P. aeruginosa infection in a murine model. However, we identified a novel role for the cytokine in promoting infection-related weight-loss, a significant prognostic factor in the CF population.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Ritchie, Dr Neil and Bayes, Dr Hannah and Evans, Professor Tom and Ward, Dr Christopher |
| Authors: | Bayes, H. K., Ritchie, N. E., Ward, C., Corris, P. A., Brodlie, M., and Evans, T. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Journal of Cystic Fibrosis |
| Publisher: | Elsevier |
| ISSN: | 1569-1993 |
| ISSN (Online): | 1873-5010 |
| Published Online: | 01 July 2016 |
| Copyright Holders: | Copyright © 2016 The Authors |
| First Published: | First published Journal of Cystic Fibrosis 2016 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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