Dynamic surfaces for the study of mesenchymal stem cell growth through adhesion regulation

Roberts, J. N. et al. (2016) Dynamic surfaces for the study of mesenchymal stem cell growth through adhesion regulation. ACS Nano, 10(7), pp. 6667-6679. (doi:10.1021/acsnano.6b01765) (PMID:27322014) (PMCID:PMC4963921)

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Out of their niche environment, adult stem cells, such as mesenchymal stem cells (MSCs), spontaneously differentiate. This makes both studying these important regenerative cells and growing large numbers of stem cells for clinical use challenging. Traditional cell culture techniques have fallen short of meeting this challenge, but materials science offers hope. In this study we have used emerging rules of managing adhesion/cytoskeletal balance to prolong MSC cultures by fabricating controllable nanoscale cell interfaces using immobilized peptides that may be enzymatically activated to change their function. The surfaces can be altered (activated) at will to tip adhesion/cytoskeletal balance and initiate differentiation hence better informing biological mechanisms of stem cell growth. Tools that are able to investigate the stem cell phenotype are important. While large phenotypical differences, such as the difference between an adipocyte and an osteoblast, are now better understood, the far more subtle differences between e.g. fibroblasts and MSCs are much harder to dissect. The development of technologies able to dynamically navigate small differences in adhesion are critical in the race to provide regenerative strategies using stem cells.

Item Type:Articles
Additional Information:Also funded by BB/G010579/1
Glasgow Author(s) Enlighten ID:Burgess, Dr Karl and McNamara, Dr Laura and Roberts, Dr Jemma and Alakpa, Miss Enateri and Dalby, Professor Matthew and Turner, Dr Lesley-Anne and Hay, Mr Jake and Yang, Dr Jingli and Yarwood, Dr Stephen and Anderson, Ms Hilary
Authors: Roberts, J. N., Sahoo, J. K., McNamara, L. E., Burgess, K. V., Yang, J., Alakpa, E. V., Anderson, H. J., Hay, J., Turner, L.-A., Yarwood, S. J., Zelzer, M., Oreffo, R. O.C., Ulijn, R. V., and Dalby, M. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:ACS Nano
Publisher:American Chemical Society
ISSN (Online):1936-086X
Published Online:20 June 2016
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in ACS Nano 10(7):66667-6679
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
608751Dynamic surfaces to mimic mesenchymal stem cell niche functionsMatthew DalbyBiotechnology and Biological Sciences Research Council (BBSRC)BB/K006908/1RI MOLECULAR CELL & SYSTEMS BIOLOGY