Posttranslational modifications of steroid receptors: phosphorylation

McGuinness, D. and McEwan, I. J. (2016) Posttranslational modifications of steroid receptors: phosphorylation. In: McEwan, I. J. (ed.) The Nuclear Receptor Superfamily: Methods and Protocols. Series: Methods in molecular biology, 1443 (1443). Springer New York, pp. 105-117. ISBN 9781493937226 (doi: 10.1007/978-1-4939-3724-0_7)

Abstract

The detection of phosphorylation status of proteins has become a critical component of the analysis of activity, localization, and turnover studies of most proteins, particularly for those involved in signaling. The androgen receptor is no exception to this rule with its localization, transcriptional activity, and interactions determined by a series of key phosphorylations on serine residues. Here we have presented a series of techniques for the investigation of the phosphorylation status and intracellular localization of the androgen receptor after hormone and growth factor stimulation of cells in culture (in vitro) and in prostate cancer tissue (in vivo). Modified methods for immunohistochemistry, immunoblotting and immunofluorescence detection with high efficacy for the measurement and monitoring of androgen receptor are presented here alongside examples of their use.

• Item Type: Book Sections
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: McGuinness, Dr Dagmara
• Authors: McGuinness, D., and McEwan, I. J.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: Methods in Molecular Biology
• Publisher: Springer New York
• ISSN: 1064-3745
• ISSN (Online): 1940-6029
• ISBN: 9781493937226