Simplicio, J. A., Hipólito, U. V., Tavares do Vale, G., Callera, G. E., Pereira, C. A., Touyz, R. M. , de Cássia Tostes, R. and Tirapelli, C. R. (2016) Acute ethanol intake induces NAD(P)H oxidase activation and RhoA translocation in resistance arteries. Arquivos Brasileiros de Cardiologia, 107(5), pp. 427-436. (doi: 10.5935/abc.20160147) (PMID:27812679) (PMCID:PMC5137387)
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Abstract
Background: The mechanism underlying the vascular dysfunction induced by ethanol is not totally understood. Identification of biochemical/molecular mechanisms that could explain such effects is warranted. Objective: To investigate whether acute ethanol intake activates the vascular RhoA/Rho kinase pathway in resistance arteries and the role of NAD(P)H oxidase-derived reactive oxygen species (ROS) on such response. We also evaluated the requirement of p47phox translocation for ethanol-induced NAD(P)H oxidase activation. Methods: Male Wistar rats were orally treated with ethanol (1g/kg, p.o. gavage) or water (control). Some rats were treated with vitamin C (250 mg/kg, p.o. gavage, 5 days) before administration of water or ethanol. The mesenteric arterial bed (MAB) was collected 30 min after ethanol administration. Results: Vitamin C prevented ethanol-induced increase in superoxide anion (O2 - ) generation and lipoperoxidation in the MAB. Catalase and superoxide dismutase activities and the reduced glutathione, nitrate and hydrogen peroxide (H2 O2 ) levels were not affected by ethanol. Vitamin C and 4-methylpyrazole prevented the increase on O2 - generation induced by ethanol in cultured MAB vascular smooth muscle cells. Ethanol had no effect on phosphorylation levels of protein kinase B (Akt) and eNOS (Ser1177 or Thr495 residues) or MAB vascular reactivity. Vitamin C prevented ethanol-induced increase in the membrane: cytosol fraction ratio of p47phox and RhoA expression in the rat MAB. Conclusion: Acute ethanol intake induces activation of the RhoA/Rho kinase pathway by a mechanism that involves ROS generation. In resistance arteries, ethanol activates NAD(P)H oxidase by inducing p47phox translocation by a redox-sensitive mechanism. (Arq Bras Cardiol. 2016; 107(5):427-436)
Item Type: | Articles |
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Additional Information: | This study was funded by Fundação de Amparo à Pesquisa do Estado de São Paulo. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Touyz, Professor Rhian |
Authors: | Simplicio, J. A., Hipólito, U. V., Tavares do Vale, G., Callera, G. E., Pereira, C. A., Touyz, R. M., de Cássia Tostes, R., and Tirapelli, C. R. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health |
Journal Name: | Arquivos Brasileiros de Cardiologia |
Journal Abbr.: | Arq Bras Cardiol |
Publisher: | Sociedade Brasileira de Cardiologia |
ISSN: | 0066-782X |
ISSN (Online): | 1678-4170 |
Copyright Holders: | Copyright © 2016 The Authors |
First Published: | First published in Arquivos Brasileiros de Cardiologia 107(5): 427-436 |
Publisher Policy: | Reproduced under a Creative Commons License |
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