Varicella zoster virus infection of human fetal lung cells alters mitochondrial morphology

Keller, A. C. et al. (2016) Varicella zoster virus infection of human fetal lung cells alters mitochondrial morphology. Journal of Neurovirology, 22(5), pp. 674-682. (doi: 10.1007/s13365-016-0457-0) (PMID:27245593)

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Abstract

Varicella zoster virus (VZV) is a ubiquitous alphaherpesvirus that establishes latency in ganglionic neurons throughout the neuraxis after primary infection. Here, we show that VZV infection induces a time-dependent significant change in mitochondrial morphology, an important indicator of cellular health, since mitochondria are involved in essential cellular functions. VZV immediate-early protein 63 (IE63) was detected in mitochondria-rich cellular fractions extracted from infected human fetal lung fibroblasts (HFL) by Western blotting. IE63 interacted with cytochrome c oxidase in bacterial 2-hybrid analyses. Confocal microscopy of VZV-infected HFL cells at multiple times after infection revealed the presence of IE63 in the nucleus, mitochondria, and cytoplasm. Our data provide the first evidence that VZV infection induces alterations in mitochondrial morphology, including fragmentation, which may be involved in cellular damage and/or death during virus infection.

Item Type:Articles
Additional Information:This work was supported by Public Health Service grants AG093716 (D.G.), AG032958 (D.G. and R.J.C.), and NS082228 (R.J.C.) from the National Institutes of Health, and VA Merit grant, CCTSI (UL1RR025780) and the Center for Women’s Health Research (J.E.B.R.). Drs. H. Badani and N. L. Baird were supported by training grant NS007321 to Dr. Gilden from the National Institutes of Health. Dr. Keller was supported by training fellowship 1P01HL14985 from the National Institutes of Health. Mr. McClatchey is supported by a VA Merit grant (J.E.B.R.).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Kennedy, Professor Peter
Authors: Keller, A. C., Badani, H., McClatchey, P. M., Baird, N. L., Bowlin, J. L., Bouchard, R., Perng, G.-C., Reusch, J. E. B., Kaufer, B. B., Gilden, D., Shahzad, A., Kennedy, P. G. E., and Cohrs, R. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Neurovirology
Publisher:Springer US
ISSN:1355-0284
ISSN (Online):1538-2443
Published Online:31 May 2016

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