Dielectrophoresis based discrimination of human embryonic stem cells from differentiating derivatives

Velugotla, S. , Pells, S., Mjoseng, H. K., Duffy, C. R.E., Smith, S., De Sousa, P. and Pethig, R. (2012) Dielectrophoresis based discrimination of human embryonic stem cells from differentiating derivatives. Biomicrofluidics, 6(4), 044113. (doi: 10.1063/1.4771316) (PMID:24339846) (PMCID:PMC3555604)

Abstract

Assessment of the dielectrophoresis(DEP) cross-over frequency (f xo), cell diameter, and derivative membranecapacitance (C m) values for a group of undifferentiated human embryonic stem cell (hESC) lines (H1, H9, RCM1, RH1), and for a transgenic subclone of H1 (T8) revealed that hESC lines could not be discriminated on their mean f xo and C m values, the latter of which ranged from 14 to 20 mF/m2. Differentiation of H1 and H9 to a mesenchymal stem cell-like phenotype resulted in similar significant increases in mean C m values to 41–49 mF/m2 in both lines (p < 0.0001). BMP4-induced differentiation of RCM1 to a trophoblast cell-like phenotype also resulted in a distinct and significant increase in mean C m value to 28 mF/m2 (p < 0.0001). The progressive transition to a higher membranecapacitance was also evident after each passage of cell culture as H9 cells transitioned to a mesenchymal stem cell-like state induced by growth on a substrate of hyaluronan. These findings confirm the existence of distinctive parameters between undifferentiated and differentiating cells on which future application of dielectrophoresis in the context of hESC manufacturing can be based.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Velugotla, Dr Srinivas
• Authors: Velugotla, S., Pells, S., Mjoseng, H. K., Duffy, C. R.E., Smith, S., De Sousa, P., and Pethig, R.
• College/School: College of Science and Engineering > School of Engineering > Electronics and Nanoscale Engineering
• Journal Name: Biomicrofluidics
• Publisher: American Institute of Physics
• ISSN: 1932-1058
• ISSN (Online): 1932-1058
• Copyright Holders: Copyright © 2012 American Institute of Physics
• First Published: First published in Biomicrofluidics 6(4):044113
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher