Molecular composition of GAG-collagen I multilayers affects remodeling of terminal layers and osteogenic differentiation of adipose-derived stem cells

Zhao, M., Altankov, G., Grabiec, U., Bennett, M., Salmeron-Sanchez, M. , Dehghani, F. and Groth, T. (2016) Molecular composition of GAG-collagen I multilayers affects remodeling of terminal layers and osteogenic differentiation of adipose-derived stem cells. Acta Biomaterialia, 41, pp. 86-99. (doi:10.1016/j.actbio.2016.05.023) (PMID:27188244)

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Abstract

The effect of molecular composition of multilayers, by pairing type I collagen (Col I) with either hyaluronic acid (HA) or chondroitin sulfate (CS) was studied regarding the osteogenic differentiation of adhering human adipose-derived stem cells (hADSCs). Multilayer (PEM) formation was based primarily on ion pairing and on additional intrinsic cross-linking through imine bond formation replacing native by oxidized HA (oHA) or CS (oCS) with Col I. Significant amounts of Col I fibrils were found on both native and oxidized CS-based PEMs, resulting in higher water contact angles and surface potential under physiological condition, while much less organized Col I was detected in either HA-based multilayers, which were more hydrophilic and negatively charged. An important finding was that hADSCs remodeled Col I at the terminal layers of PEMs by mechanical reorganization and pericellular proteolytic degradation, being more pronounced on CS-based PEMs. This was in accordance with the higher quantity of Col I deposition in this system, accompanied by more cell spreading, focal adhesions (FA) formation and significant α2β1 integrin recruitment compared to HA-based PEMs. Both CS-based PEMs caused also an increased fibronectin (FN) secretion and cell growth. Furthermore, significant calcium phosphate deposition, enhanced ALP, Col I and Runx2 expression were observed in hADSCs on CS-based PEMs, particularly on oCS-containing one. Overall, multilayer composition can be used to direct cell-matrix interactions, and hence stem cell fates showing for the first time that PEMs made of biogenic polyelectrolytes undergo significant remodeling of terminal protein layers, which enables cells to form a more adequate extracellular matrix-like environment.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel and Bennett, Mark
Authors: Zhao, M., Altankov, G., Grabiec, U., Bennett, M., Salmeron-Sanchez, M., Dehghani, F., and Groth, T.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Acta Biomaterialia
Publisher:Elsevier
ISSN:1742-7061
ISSN (Online):1878-7568
Published Online:14 May 2016
Copyright Holders:Copyright © 2016 Elsevier
First Published:First published in Act Biomaterialia 41: 86-99
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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