Genomic insights into a new acidophilic, copper-resistant Desulfosporosinus isolate from the oxidized tailings area of an abandoned gold mine

Mardanov, A. V. et al. (2016) Genomic insights into a new acidophilic, copper-resistant Desulfosporosinus isolate from the oxidized tailings area of an abandoned gold mine. FEMS Microbiology Ecology, 92(8), fiw111. (doi:10.1093/femsec/fiw111) (PMID:27222219)

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Abstract

Microbial sulfate reduction in acid mine drainage is still considered to be confined to anoxic conditions, although several reports have shown that sulfate-reducing bacteria (SRB) occur under microaerophilic or aerobic conditions. We have measured sulfate reduction rates of up to 60 nmol S cm−3day−1 in oxidized layers of gold mine tailings in Kuzbass (SW Siberia). A novel, acidophilic, copper-tolerant Desulfosporosinus sp. I2 was isolated from the same sample and its genome was sequenced. The genomic analysis and physiological data indicate the involvement of transporters and additional mechanisms to tolerate metals, such as sequestration by polyphosphates. Desulfosporinus sp. I2 encodes systems for a metabolically versatile life style. The genome possessed a complete Embden-Meyerhof pathway for glycolysis and gluconeogenesis. Complete oxidation of organic substrates could be enabled by the complete TCA cycle. Genomic analysis found all major components of the electron transfer chain necessary for energy generation via oxidative phosphorylation. Autotrophic CO2 fixation could be performed through the Wood-Ljungdahl pathway. Multiple oxygen detoxification systems were identified in the genome. Taking into account the metabolic activity and genomic analysis, the traits of the novel isolate broaden our understanding of active sulfate reduction and associated metabolism beyond strictly anaerobic niches.

Item Type:Articles
Keywords:Acidophilic sulfate-reducing bacteria, metal resistance, polyphosphates, Desulfosporosinus.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Banks, Mr David
Authors: Mardanov, A. V., Panova, I. A., Beletsky, A. V., Avakyan, M. R., Kadnikov, V. V., Antsiferov, D. V., Banks, D., Frank, Y. A., Pimenov, N. V., Ravin, N. V., and Karnachuk, O. V.
Subjects:Q Science > QR Microbiology
T Technology > TN Mining engineering. Metallurgy
College/School:College of Science and Engineering > School of Engineering > Systems Power and Energy
Journal Name:FEMS Microbiology Ecology
Publisher:Oxford University Press
ISSN:1574-6941
ISSN (Online):1574-6941
Published Online:23 May 2016
Copyright Holders:Copyright © 2016 Oxford University Press
First Published:First published in FEMS Microbiology Ecology 92(8): fiw111
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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