Characterization of G12 sandwich ELISA, a next-generation immunoassay for gluten toxicity

Halbmayr-Jech, E., Hammer, E., Fielder, R., Coutts, J., Rogers, A. and Cornish, M. (2012) Characterization of G12 sandwich ELISA, a next-generation immunoassay for gluten toxicity. Journal of AOAC International, 95(2), pp. 372-376. (doi: 10.5740/jaoacint.SGE_Halbmayr-Jech) (PMID:22649921)

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Abstract

In this work, a monoclonal antibody called G12, raised against the most immunotoxic peptide to celiac disease patients, was used to develop a sandwich ELISA. Preliminary results on cross-reactivities, recoveries, and extraction methods of the new assay are presented. The assay calibration was performed using material from the Prolamin Working Group. The antibody's specificity was determined by cross-reactivity studies on different grains, nuts, oils, and starches. Recovery of the assay was determined by spiking experiments on common food matrixes, as well as on problematic matrixes. Furthermore, sample extraction methods using ethanol, cocktail solution, and a proprietary buffer have been compared.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Coutts, Dr Jacqueline
Authors: Halbmayr-Jech, E., Hammer, E., Fielder, R., Coutts, J., Rogers, A., and Cornish, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Journal of AOAC International
Publisher:AOAC International
ISSN:1060-3271
ISSN (Online):1944-7922

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