Long-term IgG response to porcine Neu5Gc antigens without transmission of PERV in burn patients treated with porcine skin xenografts

Scobie, L. et al. (2013) Long-term IgG response to porcine Neu5Gc antigens without transmission of PERV in burn patients treated with porcine skin xenografts. Journal of Immunology, 191(6), pp. 2907-2915. (doi: 10.4049/jimmunol.1301195) (PMID:23945141) (PMCID:PMC3782708)

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Abstract

Acellular materials of xenogenic origin are used worldwide as xenografts, and phase I trials of viable pig pancreatic islets are currently being performed. However, limited information is available on transmission of porcine endogenous retrovirus (PERV) after xenotransplantation and on the long-term immune response of recipients to xenoantigens. We analyzed the blood of burn patients who had received living pig-skin dressings for up to 8 wk for the presence of PERV as well as for the level and nature of their long term (maximum, 34 y) immune response against pig Ags. Although no evidence of PERV genomic material or anti-PERV Ab response was found, we observed a moderate increase in anti-αGal Abs and a high and sustained anti–non-αGal IgG response in those patients. Abs against the nonhuman sialic acid Neu5Gc constituted the anti–non-αGal response with the recognition pattern on a sialoglycan array differing from that of burn patients treated without pig skin. These data suggest that anti-Neu5Gc Abs represent a barrier for long-term acceptance of porcine xenografts. Because anti-Neu5Gc Abs can promote chronic inflammation, the long-term safety of living and acellular pig tissue implants in recipients warrants further evaluation.

Item Type:Articles
Additional Information:Supported by European Commission Sixth Framework Programme for Research and Technological Development (FP6)-funded Project LSHB-CT-2006-037377 (to all groups in Europe), National Institutes of Health Grant GM32373 (to A.V.), and an International Sepharadic Education Foundation fellowship (to V.P.K.).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hector, Dr Ralph and Scobie, Dr Linda and Crossan, Miss Claire
Authors: Scobie, L., Padler-Karavani, V., Le Bas-Bernardet, S., Crossan, C., Blaha, J., Matouskova, M., Hector, R. D., Cozzi, E., Vanhove, B., Charreau, B., Blancho, G., Bourdais, L., Tallacchini, M., Ribes, J. M., Yu, H., Chen, X., Kracikova, J., Broz, L., Hejnar, J., Vesely, P., Takeuchi, Y., Varki, A., and Soulillou, J.-P.
College/School:College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience
Journal Name:Journal of Immunology
Publisher:American Association of Immunologists
ISSN:0022-1767
ISSN (Online):1550-6606

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
422181XENOME - Super-engineering of the porcine genome for xenotransplantation studies in primates: a step towards clinical applicationLinda ScobieEuropean Commission (EC)LSHB-CT-2006-03SCHOOL OF VETERINARY MEDICINE