Anti-envelope antibody responses in individuals at high risk of hepatitis C virus who resist infection

Swann, R.E., Mandalou, P., Robinson, M.W., Ow, M.M., Foung, S.K.H., McLauchlan, J., Patel, A.H. and Cramp, M.E. (2016) Anti-envelope antibody responses in individuals at high risk of hepatitis C virus who resist infection. Journal of Viral Hepatitis, 23(11), pp. 873-880. (doi:10.1111/jvh.12568) (PMID:27405885) (PMCID:PMC5244678)

[img]
Preview
Text
119396.pdf - Published Version
Available under License Creative Commons Attribution.

553kB

Abstract

Summary: Injection drug users uninfected by hepatitis C virus (HCV) despite likely repeated exposure through high-risk behaviour are well documented. Factors preventing infection in these individuals are incompletely understood. Here, we looked for anti-HCV-envelope antibody responses in a cohort of repeatedly exposed but uninfected subjects. Forty-two hepatitis C diagnostic antibody- and RNA-negative injection drug users at high risk of exposure were studied and findings compared to healthy controls and cases with chronic HCV infection. Purified IgGs from sera were tested by ELISA for binding to genotype 1a and 3a envelope glycoproteins E1E2 with further testing for IgG and IgM reactivity against soluble E2. Virus-neutralizing activity was assessed using an HCV pseudoparticle system. Uninfected subjects demonstrated significantly greater IgG and IgM reactivities to envelope glycoproteins than healthy controls with IgG from 6 individuals additionally showing significant neutralization. This study is the first to describe humoral immunological responses targeting the HCV envelope, important for viral neutralization, in exposed uninfected individuals. A subset of these cases also had evidence of viral neutralization via anti-envelope antibodies. In addition to confirming viral exposure, the presence of specific anti-envelope antibodies may be a factor that helps these individuals resist HCV infection.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Robinson, Dr Mark and Swann, Rachael and Patel, Professor Arvind and McLauchlan, Professor John
Authors: Swann, R.E., Mandalou, P., Robinson, M.W., Ow, M.M., Foung, S.K.H., McLauchlan, J., Patel, A.H., and Cramp, M.E.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Journal of Viral Hepatitis
Publisher:Wiley
ISSN:1352-0504
ISSN (Online):1365-2893
Published Online:13 July 2016
Copyright Holders:Copyright © 2016 Wiley
First Published:First published in Journal of Viral Hepatitis 23(11):873-880
Publisher Policy:Reproduced under a creative commons license

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
656491Basis of the host range and tissue tropism for hepatitis C virusArvind PatelMedical Research Council (MRC)MC_UU_12014/2MVLS III - CENTRE FOR VIRUS RESEARCH
501441Centre for Integrated VirologyMassimo PalmariniMedical Research Council (MRC)G0801822MVLS III - CENTRE FOR VIRUS RESEARCH