Differentiation of human mesenchymal stem cells towards quality cartilage using fibrinogen based nanofibers

Forget, J., Awaja, F., Gugutkov, D., Gustavsson, J., Gallego Ferre, G., Coelho-Sampaio, T., Hochman-Mendez, C., Salmeron-Sanchez, M. and Altankov, G. (2016) Differentiation of human mesenchymal stem cells towards quality cartilage using fibrinogen based nanofibers. Macromolecular Bioscience, 16(9), pp. 1348-1359. (doi:10.1002/mabi.201600080) (PMID:27276166)

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Abstract

Mimicking the complex intricacies of the extra cellular matrix (ECM) including three dimensional configurations and aligned fibrous structures were traditionally perused for producing cartilage tissue from stem cells. Here, we show that human adipose-derived mesenchymal stem cells (hADMSCs) established significant chondrogenic differentiation and may generate quality cartilage when cultured on two dimensional (2D) and randomly oriented fibrinogen/poly-lactic acid (FBG/PLA) nanofibers compared to a three dimensional (3D) sandwich-like environments. The adhering cells showed well-developed focal adhesion complexes and actin cytoskeleton arrangements confirming the proper cellular interaction with either random or aligned nanofibers. However, quantitative reverse transcriptionpolymerase chain reaction analysis for Collagen 2 and Collagen 10 genes expression confirmed favourable chondrogenic response of hADMSCs on random nanofibers and showed substantially higher efficacy of their differentiation in 2D configuration versus 3D constructs. These findings introduce a new direction for cartilage tissue engineering through providing a simple platform for the routine generation of transplantable stem cells-derived articular cartilage replacement that might improve joint function.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel
Authors: Forget, J., Awaja, F., Gugutkov, D., Gustavsson, J., Gallego Ferre, G., Coelho-Sampaio, T., Hochman-Mendez, C., Salmeron-Sanchez, M., and Altankov, G.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Macromolecular Bioscience
Publisher:Wiley
ISSN:1616-5187
ISSN (Online):1616-5195
Published Online:08 June 2016

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