Gelatin-hyaluronic acid hydrogels with tuned stiffness to counterbalance cellular forces and promote cell differentiation

Poveda-Reyes, S., Moulisova, V., Sanmartín-Masiá, E., Quintanilla-Sierra, L., Salmeron-Sanchez, M. and Gallego Ferrer, G. (2016) Gelatin-hyaluronic acid hydrogels with tuned stiffness to counterbalance cellular forces and promote cell differentiation. Macromolecular Bioscience, 16(9), pp. 1311-1324. (doi:10.1002/mabi.201500469) (PMID:27213762)

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Abstract

Cells interact mechanically with their environment, exerting mechanical forces that probe the extracellular matrix (ECM). The mechanical properties of the ECM determine cell behavior and control cell differentiation both in 2D and 3D environments. Gelatin is a soft hydrogel into which cells can be embedded. We show significant 3D gelatin shrinking due to the high traction cellular forces exerted by the cells on the matrix, which prevents cell differentiation. To modulate this process, we have combined Gelatin (Gel) with hyaluronic acid (HA) in an injectable crosslinked hydrogel with controlled Gel-HA ratio. HA increases matrix stiffness. The addition of small amounts of HA leads to a significant reduction in hydrogel shrinking after cell encapsulation (C2C12 myoblasts). We show that hydrogel stiffness counterbalanced traction forces of cells and this was decisive in promoting cell differentiation and myotube formation of C2C12 encapsulated in the hybrid hydrogels

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel and Moulisova, Dr Vladimira
Authors: Poveda-Reyes, S., Moulisova, V., Sanmartín-Masiá, E., Quintanilla-Sierra, L., Salmeron-Sanchez, M., and Gallego Ferrer, G.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Macromolecular Bioscience
Publisher:Wiley
ISSN:1616-5187
ISSN (Online):1616-5195
Published Online:23 May 2016
Copyright Holders:Copyright © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
First Published:First published in Macromolecular Bioscience 16(9): 1311-1324
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
626901HEALINSYNERGY - Material-driven fibronectin fibrillogenesis to engineer synergistic growth factor microenvironmentsManuel Salmeron-SanchezEuropean Research Council (ERC)306990ENG - BIOMEDICAL ENGINEERING