Prevalence and sequence-based identity of rumen fluke in cattle and deer in New Caledonia

Cauquil, L., Hüe, T., Hurlin, J.-C., Mitchell, G., Searle, K., Skuce, P. and Zadoks, R. (2016) Prevalence and sequence-based identity of rumen fluke in cattle and deer in New Caledonia. PLoS ONE, 11(4), e0152603. (doi: 10.1371/journal.pone.0152603) (PMID:27043709) (PMCID:PMC4820108)

Abstract

An abattoir survey was performed in the French Melanesian archipelago of New Caledonia to determine the prevalence of paramphistomes in cattle and deer and to generate material for molecular typing at species and subspecies level. Prevalence in adult cattle was high at animal level (70% of 387 adult cattle) and batch level (81%). Prevalence was lower in calves at both levels (33% of 484 calves, 51% at batch level). Animals from 2 of 7 deer farms were positive for rumen fluke, with animal-level prevalence of 41.4% (29/70) and 47.1% (33/70), respectively. Using ITS-2 sequencing, 3 species of paramphistomes were identified, i.e. Calicophoron calicophorum, Fischoederius elongatus and Orthocoelium streptocoelium. All three species were detected in cattle as well as deer, suggesting the possibility of rumen fluke transmission between the two host species. Based on heterogeneity in ITS-2 sequences, the C. calicophorum population comprises two clades, both of which occur in cattle as well as deer. The results suggest two distinct routes of rumen fluke introduction into this area. This approach has wider applicability for investigations of the origin of rumen fluke infections and for the possibility of parasite transmission at the livestock-wildlife interface.

• Item Type: Articles
• Additional Information: The authors would like to acknowledge financial support from the Biotechnological and Biological Sciences Research Council Industrial Partnership Award (BBSRC-IPA BB/K015591/1; KS) and the Scottish Government's Rural and Environment Science and Analytical Services Division (RESAS; GM, PJS and RNZ).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Zadoks, Professor Ruth
• Authors: Cauquil, L., Hüe, T., Hurlin, J.-C., Mitchell, G., Searle, K., Skuce, P., and Zadoks, R.
• College/School: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: PLoS ONE
• Publisher: Public Library of Science
• ISSN: 1932-6203
• ISSN (Online): 1932-6203
• Copyright Holders: Copyright © 2016 Cauquil et al.
• First Published: First published in PLoS ONE 11(4): e0152603
• Publisher Policy: Reproduced under a Creative Commons License