Vance, S. J., Desai, V., Smith, B. O. , Kennedy, M. W. and Cooper, A. (2016) Aqueous solubilization of C60 fullerene by natural protein surfactants, latherin and ranaspumin-2. Biophysical Chemistry, 214-15, pp. 27-32. (doi: 10.1016/j.bpc.2016.05.003) (PMID:27214760) (PMCID:PMC4906151)
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Abstract
C60 fullerene is not soluble in water and dispersion usually requires organic solvents, sonication or vigorous mechanical mixing. However, we show here that mixing of pristine C60 in water with natural surfactant proteins latherin and ranaspumin-2 (Rsn-2) at low concentrations yields stable aqueous dispersions with spectroscopic properties similar to those previously obtained by more vigorous methods. Particle sizes are significantly smaller than those achieved by mechanical dispersion alone, and concentrations are compatible with clusters approximating 1:1 protein:C60 stoichiometry. These proteins can also be adsorbed onto more intractable carbon nanotubes. This promises to be a convenient way to interface a range of hydrophobic nanoparticles and related materials with biological macromolecules, with potential to exploit the versatility of recombinant protein engineering in the development of nano-bio interface devices. It also has potential consequences for toxicological aspects of these and similar nanoparticles.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Kennedy, Professor Malcolm and Cooper, Professor Alan and Smith, Dr Brian |
Authors: | Vance, S. J., Desai, V., Smith, B. O., Kennedy, M. W., and Cooper, A. |
College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Science and Engineering > School of Chemistry College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
Journal Name: | Biophysical Chemistry |
Publisher: | Elsevier BV |
ISSN: | 0301-4622 |
ISSN (Online): | 1873-4200 |
Published Online: | 13 May 2016 |
Copyright Holders: | Copyright © 2016 The Authors |
First Published: | First published in Biophysical Chemistry 214-215:27-32 |
Publisher Policy: | Reproduced under a Creative Commons License |
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