Maiques, E., Quiles-Puchalt, N., Donderis, J., Ciges-Tomas, J. R., Alite, C., Bowring, J. Z. , Humphrey, S. , Penadés, J. R. and Marina, A. (2016) Another look at the mechanism involving trimeric dUTPases in Staphylococcus aureus pathogenicity island induction involves novel players in the party. Nucleic Acids Research, 44(11), pp. 5457-5469. (doi: 10.1093/nar/gkw317) (PMID:27112567) (PMCID:PMC4914113)
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Abstract
We have recently proposed that the trimeric staphylococcal phage encoded dUTPases (Duts) are signaling molecules that act analogously to eukaryotic G-proteins, using dUTP as a second messenger. To perform this regulatory role, the Duts require their characteristic extra motif VI, present in all the staphylococcal phage coded trimeric Duts, as well as the strongly conserved Dut motif V. Recently, however, an alternative model involving Duts in the transfer of the staphylococcal islands (SaPIs) has been suggested, questioning the implication of motifs V and VI. Here, using state-of the-art techniques, we have revisited the proposed models. Our results confirm that the mechanism by which the Duts derepress the SaPI cycle depends on dUTP and involves both motifs V and VI, as we have previously proposed. Surprisingly, the conserved Dut motif IV is also implicated in SaPI derepression. However, and in agreement with the proposed alternative model, the dUTP inhibits rather than inducing the process, as we had initially proposed. In summary, our results clarify, validate and establish the mechanism by which the Duts perform regulatory functions.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Bowring, Janine Zara and Penades, Prof Jose R and Humphrey, Dr Suzie and Quiles, Miss Nuria |
Authors: | Maiques, E., Quiles-Puchalt, N., Donderis, J., Ciges-Tomas, J. R., Alite, C., Bowring, J. Z., Humphrey, S., Penadés, J. R., and Marina, A. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Nucleic Acids Research |
Publisher: | Oxford University Press |
ISSN: | 0305-1048 |
ISSN (Online): | 1362-4962 |
Published Online: | 25 April 2016 |
Copyright Holders: | Copyright © 2016 The Authors |
First Published: | First published in Nucleic Acids Research 44(11):5457-5469 |
Publisher Policy: | Reproduced under a Creative Commons License |
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