PLLA/ZnO nanocomposites: dynamic surfaces to harness cell differentiation

Trujillo, S. , Lizundia, E., Vilas, J. L. and Salmeron-Sanchez, M. (2016) PLLA/ZnO nanocomposites: dynamic surfaces to harness cell differentiation. Colloids and Surfaces B: Biointerfaces, 144, pp. 152-160. (doi:10.1016/j.colsurfb.2016.04.007)

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Abstract

This work investigates the effect of the sequential availability of ZnO nanoparticles, (nanorods of ∼40 nm) loaded within a degradable poly(lactic acid) (PLLA) matrix, in cell differentiation. The system constitutes a dynamic surface, in which nanoparticles are exposed as the polymer matrix degrades. ZnO nanoparticles were loaded into PLLA and the system was measured at different time points to characterise the time evolution of the physicochemical properties, including wettability and thermal properties. The micro and nanostructure were also investigated using AFM, SEM and TEM images. Cellular experiments with C2C12 myoblasts show that cell differentiation was significantly enhanced on ZnO nanoparticles—loaded PLLA, as the polymer degrades and the availability of nanoparticles become more apparent, whereas the release of zinc within the culture medium was negligible. Our results suggest PLLA/ZnO nanocomposites can be used as a dynamic system where nanoparticles are exposed during degradation, activating the material surface and driving cell differentiation.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel and Trujillo Munoz, Dr Sara
Authors: Trujillo, S., Lizundia, E., Vilas, J. L., and Salmeron-Sanchez, M.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Colloids and Surfaces B: Biointerfaces
Publisher:Elsevier
ISSN:0927-7765
ISSN (Online):1873-4367
Copyright Holders:Copyright © 2016 Elsevier B. V.
First Published:First published in Colloids and Surfaces B: Biointerfaces 144:152-160
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
626901HEALINSYNERGY - Material-driven fibronectin fibrillogenesis to engineer synergistic growth factor microenvironmentsManuel Salmeron-SanchezEuropean Research Council (ERC)306990ENG - BIOMEDICAL ENGINEERING