The importance of lag time extension in determining bacterial resistance to antibiotics

Li, B., Qiu, Y., Shi, H. and Yin, H. (2016) The importance of lag time extension in determining bacterial resistance to antibiotics. Analyst, 141(10), pp. 3059-3067. (doi: 10.1039/C5AN02649K) (PMID:27077143)

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It is widely appreciated that widespread antibiotic resistance has significantly reduced the utility of today’s antibiotics. Many antibiotics now fail to cure infectious diseases, although they are classified as effective bactericidal agents based on antibiotic susceptibility tests. Here, via kinetic growth assays, we evaluated the effects of 12 commonly used antibiotics on the lag phase of a range of pure environmental isolates and sludge bacterial communities of high diversity. We show that extended lag phase offers bacteria survival advantages and promotes regrowth upon the removal of antibiotics. By utilizing both lag phase extension and IC50, the killing efficiency of an antibiotic on a strain or a community can be easily revealed. Interestingly, for several strains of relevance to endemic nosocomial infections (e.g. Acinetobacter sp. and Pseudomonas aeruginosa) and the diverse communities, tetracycline and quinolone antibiotics are most likely to be resisted via extended lag phase. This discovery is significant from a clinical point view since underestimation of bacteria resistance can lead to the recurrence of diseases.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Yin, Professor Huabing
Authors: Li, B., Qiu, Y., Shi, H., and Yin, H.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Analyst
Publisher:Royal Society of Chemistry
ISSN (Online):1364-5528
Published Online:07 April 2016
Copyright Holders:Copyright © 2016 The Royal Society of Chemistry
First Published:First published in Analyst 141(10): 3059-3067
Publisher Policy:Reproduced under a Creative Commons License
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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
532403Engineering a semi-biotic systemHuabing YinEngineering & Physical Sciences Research Council (EPSRC)EP/H04986X/1ENG - BIOMEDICAL ENGINEERING