Genetic vaccination against leishmaniasis

Xu, D. and Liew, F.Y. (1994) Genetic vaccination against leishmaniasis. Vaccine, 12(16), pp. 1534-1536. (doi:10.1016/0264-410X(94)90079-5) (PMID:7879420)

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Abstract

The gene encoding for a major surface glycoprotein, gp63, of Leishmania major was cloned into the eukaryotic expression plasmid pCDNAI with CMV or RSV promoters. The highly susceptible Balb/c mice were injected intramuscularly with 100 μg/mouse of the purified plasmid. The plasmids were found to be stable in vivo for at least 40 days after injection and expressed significant levels of gp63, demonstrable by immunohistological staining with specific antibody. The immunized mice developed significant resistance against L. major infection compared to controls similarly immunized with the empty plasmid. Spleen cells from the immunized mice produced significant levels of IL-2 and IFN-γ but no detectable IL-4 when cultured with leishmanial antigens in vitro.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Liew, Professor Foo and Xu, Dr Damo
Authors: Xu, D., and Liew, F.Y.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Vaccine
Publisher:Elsevier
ISSN:0264-410X
ISSN (Online):1873-2518

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