Perrella, G., Carr, C., Asensi-Fabado, M. A., Donald, N. A., Páldi, K., Hannah, M. A. and Amtmann, A. (2016) The Histone Deacetylase Complex (HDC) 1 protein of Arabidopsis thaliana has the capacity to interact with multiple proteins including histone 3-binding proteins and histone 1 variants. Plant Physiology, 171(1), pp. 62-70. (doi: 10.1104/pp.15.01760) (PMID:26951436) (PMCID:PMC4854681)
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Abstract
Intrinsically disordered proteins can adopt multiple conformations thereby enabling interaction with a wide variety of partners. They often serve as hubs in protein interaction networks. We have previously shown that the Histone Deacetylase Complex (HDC) 1 protein from Arabidopsis thaliana interacts with histone deacetylases and quantitatively determines histone acetylation levels, transcriptional activity and several phenotypes, including ABA-sensitivity during germination, vegetative growth rate and flowering time. HDC1-type proteins are ubiquitous in plants but they contain no known structural or functional domains. Here we explored the protein interaction spectrum of HDC1. In addition to binding histone deacetylases, HDC1 directly interacted with core histone H3-binding proteins and co-repressor associated proteins, but not with H3 or the co-repressors themselves. Surprisingly, HDC1 was also able to interact with variants of the linker histone H1. Truncation of HDC1 to the ancestral core sequence narrowed the spectrum of interactions and of phenotypic outputs but maintained binding to a H3-binding protein and to H1. The results indicate a potential link between H1 and histone modifying complexes.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Carr, Mr Craig and Amtmann, Professor Anna and Perrella, Dr Giorgio and Asensi-Fabado, Ms Maria Amparo |
| Authors: | Perrella, G., Carr, C., Asensi-Fabado, M. A., Donald, N. A., Páldi, K., Hannah, M. A., and Amtmann, A. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
| Journal Name: | Plant Physiology |
| Publisher: | American Society of Plant Biologists |
| ISSN: | 0032-0889 |
| ISSN (Online): | 1532-2548 |
| Published Online: | 07 March 2016 |
| Copyright Holders: | Copyright © 2016 The American Society of Plant Biologists |
| First Published: | First published in Plant Physiology 171(1):62-70 |
| Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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