Comparative miRNA-based fingerprinting reveals biological differences in human olfactory mucosa and bone marrow-derived mesenchymal stromal cells

Lindsay, S. L. , Johnstone, S. A., Anthony, M., Mallinson, D. and Barnett, S. C. (2016) Comparative miRNA-based fingerprinting reveals biological differences in human olfactory mucosa and bone marrow-derived mesenchymal stromal cells. Stem Cell Reports, 6(5), pp. 729-742. (doi: 10.1016/j.stemcr.2016.03.009) (PMID:27117785) (PMCID:PMC4940454)

[img]
Preview
Text
118024.pdf - Published Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

4MB

Abstract

Previously we reported that nestin-positive human mesenchymal stromal cells (MSCs) derived from the olfactory mucosa (OM) enhanced CNS myelination in vitro to a greater extent than bone-marrow-derived MSCs (BM-MSCs). miRNA-based fingerprinting revealed the two MSCs were 64% homologous, with 26 miRNAs differentially expressed. We focused on miR-146a-5p and miR-140-5p due to their reported role in the regulation of chemokine production and myelination. The lower expression of miR-140-5p in OM-MSCs correlated with higher secretion of CXCL12 compared with BM-MSCs. Addition of CXCL12 and its pharmacological inhibitors to neural co-cultures supported these data. Studies on related miR-146a-5p targets demonstrated that OM-MSCs had lower levels of Toll-like receptors and secreted less pro-inflammatory cytokines, IL-6, IL-8, and CCL2. OM-MSCs polarized microglia to an anti-inflammatory phenotype, illustrating potential differences in their inflammatory response. Nestin-positive OM-MSCs could therefore offer a cell transplantation alternative for CNS repair, should these biological behaviors be translated in vivo.

Item Type:Articles
Additional Information:The work was funded as follows: Tenovus Foundation (SL), Medical Research Council (MRC) (SL, MR/J004731/1), Lord Kelvin/Adam Smith studentship from the University of Glasgow (SJ), and NC3Rs studentship (MM, NC/K50032X/1)
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Barnett, Professor Susan and Lindsay, Dr Susan and Johnstone, Dr Steven
Authors: Lindsay, S. L., Johnstone, S. A., Anthony, M., Mallinson, D., and Barnett, S. C.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Stem Cell Reports
Publisher:Elsevier Inc.
ISSN:2213-6711
ISSN (Online):2213-6711
Published Online:21 April 2016
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Stem Cell Reports 2016
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
584331Does cellular niche affect the repair potential of mesenchymal stem cells; implications for spinal cord injury?Susan BarnettMedical Research Council (MRC)MR/J004731/1III -IMMUNOLOGY