Lindsay, S. L. , Johnstone, S. A., Anthony, M., Mallinson, D. and Barnett, S. C. (2016) Comparative miRNA-based fingerprinting reveals biological differences in human olfactory mucosa and bone marrow-derived mesenchymal stromal cells. Stem Cell Reports, 6(5), pp. 729-742. (doi: 10.1016/j.stemcr.2016.03.009) (PMID:27117785) (PMCID:PMC4940454)
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Abstract
Previously we reported that nestin-positive human mesenchymal stromal cells (MSCs) derived from the olfactory mucosa (OM) enhanced CNS myelination in vitro to a greater extent than bone-marrow-derived MSCs (BM-MSCs). miRNA-based fingerprinting revealed the two MSCs were 64% homologous, with 26 miRNAs differentially expressed. We focused on miR-146a-5p and miR-140-5p due to their reported role in the regulation of chemokine production and myelination. The lower expression of miR-140-5p in OM-MSCs correlated with higher secretion of CXCL12 compared with BM-MSCs. Addition of CXCL12 and its pharmacological inhibitors to neural co-cultures supported these data. Studies on related miR-146a-5p targets demonstrated that OM-MSCs had lower levels of Toll-like receptors and secreted less pro-inflammatory cytokines, IL-6, IL-8, and CCL2. OM-MSCs polarized microglia to an anti-inflammatory phenotype, illustrating potential differences in their inflammatory response. Nestin-positive OM-MSCs could therefore offer a cell transplantation alternative for CNS repair, should these biological behaviors be translated in vivo.
Item Type: | Articles |
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Additional Information: | The work was funded as follows: Tenovus Foundation (SL), Medical Research Council (MRC) (SL, MR/J004731/1), Lord Kelvin/Adam Smith studentship from the University of Glasgow (SJ), and NC3Rs studentship (MM, NC/K50032X/1) |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Barnett, Professor Susan and Lindsay, Dr Susan and Johnstone, Dr Steven |
Authors: | Lindsay, S. L., Johnstone, S. A., Anthony, M., Mallinson, D., and Barnett, S. C. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Stem Cell Reports |
Publisher: | Elsevier Inc. |
ISSN: | 2213-6711 |
ISSN (Online): | 2213-6711 |
Published Online: | 21 April 2016 |
Copyright Holders: | Copyright © 2016 The Authors |
First Published: | First published in Stem Cell Reports 2016 |
Publisher Policy: | Reproduced under a Creative Commons License |
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