Aitken, L., Quinn, S. D. , Perez-Gonzalez, C., Samuel, I. D. W., Penedo, J. C. and Gunn-Moore, F. J. (2016) Morphology-specific inhibition of β-amyloid aggregates by 17β-hydroxysteroid dehydrogenase type 10. ChemBioChem, 17(11), pp. 1029-1037. (doi: 10.1002/cbic.201600081) (PMID:26991863)
|
Text
117674.pdf - Accepted Version 1MB |
Abstract
A major hallmark of Alzheimer's disease (AD) is the formation of toxic aggregates composed of the β-amyloid peptide (Aβ). Given that Aβ peptides are known to co-localize within mitochondria and interact with 17β-HSD10, a mitochondrial protein expressed at high levels in AD brains, we have investigated the inhibitory potential of 17β-HSD10 against Aβ aggregation across a range of physiological conditions. The fluorescence self-quenching (FSQ) of Aβ(1-42), labelled with HiLyte Fluor 555, was used as a sensing strategy to evaluate the inhibitory effect of 17β-HSD10 under well-established conditions to grow distinct Aβ morphologies. Our results indicate that 17β-HSD10 preferentially inhibits the formation of globular and fibrillar-like structures but has no effect on the growth of amorphous plaque-like aggregates at endosomal pH 6. This work provides insights into the dependence of the Aβ-17β-HSD10 interaction with the morphology of Aβ aggregates and how this impacts enzymatic function.
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Quinn, Dr Steven |
Authors: | Aitken, L., Quinn, S. D., Perez-Gonzalez, C., Samuel, I. D. W., Penedo, J. C., and Gunn-Moore, F. J. |
College/School: | College of Science and Engineering > School of Chemistry |
Journal Name: | ChemBioChem |
Publisher: | Wiley-VCH Verlag |
ISSN: | 1439-4227 |
ISSN (Online): | 1439-7633 |
Published Online: | 25 April 2016 |
Copyright Holders: | Copyright © 2016 Wiley-VCH Verlag |
First Published: | First published in ChemBioChem 17(11):1029-1037 |
Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
University Staff: Request a correction | Enlighten Editors: Update this record