Living biointerfaces based on non-pathogenic bacteria support stem cell differentiation

Hay, J. J., Rodrigo-Navarro, A., Hassi, K., Moulisova, V., Dalby, M. J. and Salmeron-Sanchez, M. (2016) Living biointerfaces based on non-pathogenic bacteria support stem cell differentiation. Scientific Reports, 6, 21809. (doi:10.1038/srep21809) (PMID:26902619) (PMCID:PMC4763179)

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Abstract

Lactococcus lactis, a non-pathogenic bacteria, has been genetically engineered to express the III7–10 fragment of human fibronectin as a membrane protein. The engineered L. lactis is able to develop biofilms on different surfaces (such as glass and synthetic polymers) and serves as a long-term substrate for mammalian cell culture, specifically human mesenchymal stem cells (hMSC). This system constitutes a living interface between biomaterials and stem cells. The engineered biofilms remain stable and viable for up to 28 days while the expressed fibronectin fragment induces hMSC adhesion. We have optimised conditions to allow long-term mammalian cell culture, and found that the biofilm is functionally equivalent to a fibronectin-coated surface in terms of osteoblastic differentiation using bone morphogenetic protein 2 (BMP-2) added to the medium. This living bacteria interface holds promise as a dynamic substrate for stem cell differentiation that can be further engineered to express other biochemical cues to control hMSC differentiation.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Moulisova, Dr Vladimira and Salmeron-Sanchez, Professor Manuel and Dalby, Professor Matthew and Rodrigo-Navarro, Mr Aleixandre
Authors: Hay, J. J., Rodrigo-Navarro, A., Hassi, K., Moulisova, V., Dalby, M. J., and Salmeron-Sanchez, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Scientific Reports
Publisher:Nature Publishing Group
ISSN:2045-2322
ISSN (Online):2045-2322
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Scientific Reports 6:21809
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
691041Living interfaces based on non-pathogenic bacterial to control stem cell differentiationManuel Salmeron-SanchezLeverhulme Trust (LEVERHULME)RPG-2015-191ENG - BIOMEDICAL ENGINEERING