Conserved and host-specific features of influenza virion architecture

Hutchinson, E. C. , Charles, P. D., Hester, S. S., Thomas, B., Trudgian, D., Martínez-Alonso, M. and Fodor, E. (2014) Conserved and host-specific features of influenza virion architecture. Nature Communications, 5, p. 4816. (doi:10.1038/ncomms5816) (PMID:25226414) (PMCID:PMC4167602)

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Abstract

Viruses use virions to spread between hosts, and virion composition is therefore the primary determinant of viral transmissibility and immunogenicity. However, the virions of many viruses are complex and pleomorphic, making them difficult to analyse in detail. Here we address this by identifying and quantifying virion proteins with mass spectrometry, producing a complete and quantified model of the hundreds of host-encoded and viral proteins that make up the pleomorphic virions of influenza viruses. We show that a conserved influenza virion architecture is maintained across diverse combinations of virus and host. This ‘core’ architecture, which includes substantial quantities of host proteins as well as the viral protein ​NS1, is elaborated with abundant host-dependent features. As a result, influenza virions produced by mammalian and avian hosts have distinct protein compositions. Finally, we note that influenza virions share an underlying protein composition with exosomes, suggesting that influenza virions form by subverting microvesicle production.

Item Type:Articles
Additional Information:The work was supported by the MRC (programme grant MR/K000241/1 to E.F.)
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hutchinson, Dr Edward
Authors: Hutchinson, E. C., Charles, P. D., Hester, S. S., Thomas, B., Trudgian, D., Martínez-Alonso, M., and Fodor, E.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Nature Communications
Publisher:Nature Publishing Group
ISSN (Online):2041-1723
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