Production of nitric oxide in the synovial membrane of rheumatoid and osteoarthritis patients

McInnes, I. B. , Leung, B. P., Field, M., Wei, X. Q., Huang, F.-P., Sturrock, R. D., Kinninmonth, A., Weidner, J., Mumford, R. and Liew, F. Y. (1996) Production of nitric oxide in the synovial membrane of rheumatoid and osteoarthritis patients. Journal of Experimental Medicine, 184(4), pp. 1519-1524. (doi:10.1084/jem.184.4.1519) (PMID:8879223) (PMCID:PMC2192822)

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Abstract

We have demonstrated spontaneous nitric oxide (NO) production by primary synovial cultures from rheumatoid (RA) and osteoarthritis patients. Increased NO production followed addition of staphylococcal enterotoxin B. Immunochemical double staining with specific anti-human inducible NO synthase (iNOS) and nonspecific esterase (NSE), or anti-CD68 (markers for tissue macrophages) showed that although many lining layer cells in RA synovium expressed iNOS, most (approximately 90%) were NSE- and CD68-, with only a minor population (approximately 10%) which were iNOS+, CD68+/NSE+. These data demonstrate the capacity for high output of NO by human synovial tissue and show that, although human macrophages can express high levels of iNOS, the majority of cells expressing iNOS are fibroblasts. We also report that synoviocytes, and macrophage cell lines, cultured with the NO donor, S-nitroso-acetyl penicillamine, produced high concentrations of tumor necrosis factor (TNF)-alpha. These results suggest that NO may mediate pathology in RA through the induction of TNF-alpha production.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Liew, Professor Foo and McInnes, Professor Iain and Sturrock, Professor Roger and Field, Dr Max and Leung, Dr Bernard
Authors: McInnes, I. B., Leung, B. P., Field, M., Wei, X. Q., Huang, F.-P., Sturrock, R. D., Kinninmonth, A., Weidner, J., Mumford, R., and Liew, F. Y.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
Journal Name:Journal of Experimental Medicine
Publisher:Rockefeller University Press
ISSN:0022-1007

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