Reactive oxygen species can provide atheroprotection via NOX4-dependent inhibition of inflammation and vascular remodelling significance

Gray, S. P. et al. (2016) Reactive oxygen species can provide atheroprotection via NOX4-dependent inhibition of inflammation and vascular remodelling significance. Arteriosclerosis, Thrombosis, and Vascular Biology, 36(2), pp. 295-307. (doi: 10.1161/atvbaha.115.307012) (PMID:26715682)

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Objective—Oxidative stress is considered a hallmark of atherosclerosis. In particular, the superoxide-generating type 1 NADPH oxidase (NOX1) has been shown to be induced and play a pivotal role in early phases of mouse models of atherosclerosis and in the context of diabetes mellitus. Here, we investigated the role of the most abundant type 4 isoform (NOX4) in human and mouse advanced atherosclerosis. Approach and Results—Plaques of patients with cardiovascular events or established diabetes mellitus showed a surprising reduction in expression of the most abundant but hydrogen peroxide (H2O2)-generating type 4 isoform (Nox4), whereas Nox1 mRNA was elevated, when compared with respective controls. As these data suggested that NOX4-derived reactive oxygen species may convey a surprisingly protective effect during plaque progression, we examined a mouse model of accelerated and advanced diabetic atherosclerosis, the streptozotocin-treated ApoE−/− mouse, with (NOX4−/−) and without genetic deletion of Nox4. Similar to the human data, advanced versus early plaques of wild-type mice showed reduced Nox4 mRNA expression. Consistent with a rather protective role of NOX4-derived reactive oxygen species, NOX4−/− mice showed increased atherosclerosis when compared with wild-type mice. Deleting NOX4 was associated with reduced H2O2 forming activity and attenuation of the proinflammatory markers, monocyte chemotratic protein-1, interleukin-1β, and tumor necrosis factor-α, as well as vascular macrophage accumulation. Furthermore, there was a greater accumulation of fibrillar collagen fibres within the vascular wall and plaque in diabetic Nox4−/−ApoE−/− mice, indicative of plaque remodeling. These data could be replicated in human aortic endothelial cells in vitro, where Nox4 overexpression increased H2O2 and reduced the expression of pro-oxidants and profibrotic markers. Interestingly, Nox4 levels inversely correlated with Nox2 gene and protein levels. Although NOX2 is not constitutively active unlike NOX4 and forms rather superoxide, this opens up the possibility that at least some effects of NOX4 deletion are mediated by NOX2 activation. Conclusions—Thus, the appearance of reactive oxygen species in atherosclerosis is apparently not always a nondesirable oxidative stress, but can also have protective effects. Both in humans and in mouse, the H2O2-forming NOX4, unlike the superoxide-forming NOX1, can act as a negative modulator of inflammation and remodeling and convey atheroprotection. These results have implications on how to judge reactive oxygen species formation in cardiovascular disease and need to be considered in the development of NOX inhibitory drugs.

Item Type:Articles
Additional Information:This work was supported by the National Health and Medical Research Council (NHMRC) project grant of Australia, the Juvenile Diabetes Research Foundation, and the Diabetes Australia Research Trust. K.A.M. Jandeleit-Dahm is supported by a NHMRC Senior Research Fellowship and M.E. Cooper is supported by an NHMRC Senior Principal Research Fellowship. S.P. Gray is supported by the Australian Diabetes Society Skip Martin Early Career Fellowship. E. Di Marco is supported by the National Heart Foundation Postgraduate Scholarship. A.C. Calkin is supported by a National Heart Foundation Future Leader Fellowship. H.H.H.W. Schmidt is supported by the EU (Marie Curie International Reintegration Grant and the ERC Advanced Grant 30983347E RADMED) and a cofounder and minor shareholder of Vasopharm GmbH, Würzburg, Germany. This study was also supported, in part, by the Victorian Government’s Operational Infrastructure Support Program.
Glasgow Author(s) Enlighten ID:Touyz, Professor Rhian
Authors: Gray, S. P., Di Marco, E., Kennedy, K., Chew, P., Okabe, J., El-Osta, A., Calkin, A. C., Biessen, E. A.L., Touyz, R. M., Cooper, M. E., Schmidt, H. H.H.W., and Jandeleit-Dahm, K. A.M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:Arteriosclerosis, Thrombosis, and Vascular Biology
Publisher:American Heart Association
ISSN (Online):1524-4636

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