The corticotropin-releasing factor-like diuretic hormone 44 (DH44) and kinin neuropeptides modulate desiccation and starvation tolerance in Drosophila melanogaster

Cannell, E., Dornan, A. J. , Halberg, K. A., Terhzaza, S. , Dow, J. A.T. and Davies, S. A. (2016) The corticotropin-releasing factor-like diuretic hormone 44 (DH44) and kinin neuropeptides modulate desiccation and starvation tolerance in Drosophila melanogaster. Peptides, 80, (doi:10.1016/j.peptides.2016.02.004) (PMID:26896569) (PMCID:PMC4889782)

[img]
Preview
Text
116539.pdf - Published Version
Available under License Creative Commons Attribution.

3MB

Abstract

Malpighian tubules are critical organs for epithelial fluid transport and stress tolerance in insects, and are under neuroendocrine control by multiple neuropeptides secreted by identified neurons. Here, we demonstrate roles for CRF-like diuretic hormone 44 (DH44) and Drosophila melanogaster kinin (Drome-kinin, DK) in desiccation and starvation tolerance. Gene expression and labelled DH44 ligand binding data, as well as highly selective knockdowns and/or neuronal ablations of DH44 in neurons of the pars intercerebralis and DH44 receptor (DH44-R2) in Malpighian tubule principal cells, indicate that suppression of DH44 signalling improves desiccation tolerance of the intact fly. Drome-kinin receptor, encoded by the leucokinin receptor gene, LKR, is expressed in DH44 neurons as well as in stellate cells of the Malpighian tubules. LKR knockdown in DH44-expressing neurons reduces Malpighian tubule-specific LKR, suggesting interactions between DH44 and LK signalling pathways. Finally, although a role for DK in desiccation tolerance was not defined, we demonstrate a novel role for Malpighian tubule cell-specific LKR in starvation tolerance. Starvation increases gene expression of epithelial LKR. Also, Malpighian tubule stellate cell-specific knockdown of LKR significantly reduced starvation tolerance, demonstrating a role for neuropeptide signalling during starvation stress.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Dow, Professor Julian and Terhzaz, Dr Selim and Davies, Professor Shireen and Halberg, Dr Kenneth and Dornan, Dr Anthony
Authors: Cannell, E., Dornan, A. J., Halberg, K. A., Terhzaza, S., Dow, J. A.T., and Davies, S. A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Peptides
Publisher:Elsevier
ISSN:0196-9781
ISSN (Online):1873-5169
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Peptides 80:96-107
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
590542Wellcome 096987 - E CannellShireen DaviesWellcome Trust (WELLCOME)096987/Z/11/ZRI MOLECULAR CELL & SYSTEMS BIOLOGY