ER stress and basement membrane defects combine to cause glomerular and tubular renal disease resulting from Col4a1 mutations in mice

Jones, F. E. et al. (2016) ER stress and basement membrane defects combine to cause glomerular and tubular renal disease resulting from Col4a1 mutations in mice. Disease Models and Mechanisms, 9(2), pp. 165-76. (doi:10.1242/dmm.021741) (PMID:26839400) (PMCID:PMC4770143)

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Abstract

Collagen IV is a major component of basement membranes, and mutations in COL4A1, which encodes collagen IV alpha chain 1, cause a multisystemic disease encompassing cerebrovascular, eye and kidney defects. However, COL4A1 renal disease remains poorly characterized and its pathomolecular mechanisms are unknown. We show that Col4a1 mutations in mice cause hypotension and renal disease, including proteinuria and defects in Bowman's capsule and the glomerular basement membrane, indicating a role for Col4a1 in glomerular filtration. Impaired sodium reabsorption in the loop of Henle and distal nephron despite elevated aldosterone levels indicates that tubular defects contribute to the hypotension, highlighting a novel role for the basement membrane in vascular homeostasis by modulation of the tubular response to aldosterone. Col4a1 mutations also cause diabetes insipidus, whereby the tubular defects lead to polyuria associated with medullary atrophy and a subsequent reduction in the ability to upregulate aquaporin 2 and concentrate urine. Moreover, haematuria, haemorrhage and vascular basement membrane defects confirm an important vascular component. Interestingly, although structural and compositional basement membrane defects occurred in the glomerulus and Bowman's capsule, no tubular basement membrane defects were detected. By contrast, medullary atrophy was associated with chronic ER stress, providing evidence for cell-type-dependent molecular mechanisms of Col4a1 mutations. These data show that both basement membrane defects and ER stress contribute to Col4a1 renal disease, which has important implications for the development of treatment strategies for collagenopathies.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Jones, Dr Frances and Van Agtmael, Dr Tom
Authors: Jones, F. E., Bailey, M. A., Murray, L. S., Lu, Y., McNeilly, S., Schlötzer-Schrehardt, U., Lennon, R., Sado, Y., Brownstein, D. G., Mullins, J. J., Kadler, K. E., and Van Agtmael, T.
Subjects:Q Science > QH Natural history > QH426 Genetics
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:Disease Models and Mechanisms
Publisher:The Company of Biologists Ltd.
ISSN:1754-8403
ISSN (Online):1754-8411
Copyright Holders:Copyright © 2016 The Company of Biologists Ltd.
First Published:First published in Disease Models and Mechanisms 9:165-176
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
471511Collagen type IV in vascular biologyTom Van AgtmaelMedical Research Council (MRC)G0601268RI CARDIOVASCULAR & MEDICAL SCIENCES
602621Elucidation of molecular pathways underlying renal disease caused by Co/4a 1 mutations using mouse models.Tom Van AgtmaelKidney Research UK (NKRF)RP19/2012RI CARDIOVASCULAR & MEDICAL SCIENCES