Extensive multiplex PCR diagnostics reveals new insights into the epidemiology of viral respiratory infections

Nickbakhsh, S. , Thorburn, F., Von Wissmann, B., McMenamin, J., Gunson, R. N. and Murcia, P. R. (2016) Extensive multiplex PCR diagnostics reveals new insights into the epidemiology of viral respiratory infections. Epidemiology and Infection, 144(10), pp. 2064-2076. (doi: 10.1017/S0950268816000339) (PMID:26931455)

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Abstract

Viral respiratory infections continue to pose a major global healthcare burden. At the community level, the co-circulation of respiratory viruses is common and yet studies generally focus on single aetiologies. We conducted the first comprehensive epidemiological analysis to encompass all major respiratory viruses in a single population. Using extensive multiplex PCR diagnostic data generated by the largest NHS board in Scotland, we analysed 44230 patient episodes of respiratory illness that were simultaneously tested for 11 virus groups between 2005 and 2013, spanning the 2009 influenza A pandemic. We measured viral infection prevalence, described co-infections, and identified factors independently associated with viral infection using multivariable logistic regression. Our study provides baseline measures and reveals new insights that will direct future research into the epidemiological consequences of virus co-circulation. In particular, our study shows that (i) human coronavirus infections are more common during influenza seasons and in co-infections than previously recognized, (ii) factors associated with co-infection differ from those associated with viral infection overall, (iii) virus prevalence has increased over time especially in infants aged <1 year, and (iv) viral infection risk is greater in the post-2009 pandemic era, likely reflecting a widespread change in the viral population that warrants further investigation.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:McMenamin, Dr James and Nickbakhsh, Dr Sema and Gunson, Dr Rory and Thorburn, Dr Fiona and Murcia, Professor Pablo
Authors: Nickbakhsh, S., Thorburn, F., Von Wissmann, B., McMenamin, J., Gunson, R. N., and Murcia, P. R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Epidemiology and Infection
Publisher:Cambridge University Press
ISSN:0950-2688
ISSN (Online):1469-4409
Published Online:02 March 2016
Copyright Holders:Copyright © 2016 Cambridge University Press
First Published:First published in Epidemiology and Infection 144(10): 2064-2076
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher.

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
501441Centre for Integrated VirologyMassimo PalmariniMedical Research Council (MRC)G0801822MVLS III - CENTRE FOR VIRUS RESEARCH