Stability of small ubiquitin-like modifier (SUMO) proteases OVERLY TOLERANT TO SALT1 and -2 modulates salicylic acid signalling and SUMO1/2 conjugation inArabidopsis thaliana

Bailey, M. et al. (2016) Stability of small ubiquitin-like modifier (SUMO) proteases OVERLY TOLERANT TO SALT1 and -2 modulates salicylic acid signalling and SUMO1/2 conjugation inArabidopsis thaliana. Journal of Experimental Botany, 67(1), pp. 353-363. (doi:10.1093/jxb/erv468) (PMID:26494731) (PMCID:PMC4682439)

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Abstract

Small ubiquitin-like modifier proteases 1 and 2 (SUMO1/2) have been linked to the regulation of salicylic acid (SA)-mediated defence signalling in Arabidopsis thaliana. In order to define the role of the SUMO proteases OVERLY TOLERANT TO SALT1 and -2 (OTS1/2) in defence and to provide insight into SUMO1/2-mediated regulation of SA signalling, we examined the status of SA-mediated defences in ots1/2 mutants. The ots1 ots2 double mutant displayed enhanced resistance to virulent Pseudomonas syringae and higher levels of SA compared with wild-type (WT) plants. Furthermore, ots1 ots2 mutants exhibited upregulated expression of the SA biosynthesis gene ICS1 in addition to enhanced SA-responsive ICS1 expression beyond that of WT. SA stimulated OTS1/2 degradation and promoted accumulation of SUMO1/2 conjugates. These results indicate that OTS1 and -2 act in a feedback loop in SA signalling and that de novo OTS1/2 synthesis works antagonistically to SA-promoted degradation, adjusting the abundance of OTS1/2 to moderate SA signalling. Accumulation of SUMO1/2 conjugates coincides with SA-promoted OTS degradation and may play a positive role in SA-mediated signalling in addition to its repressive roles reported elsewhere.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Sadanandom, Dr Ari and Conti, Dr Lucio and Milner, Dr Joel and Love, Dr Andrew
Authors: Bailey, M., Srivastava, A., Conti, L., Nelis, S., Zhang, C., Florance, H., Love, A., Milner, J., Napier, R., Grant, M., and Sadanandom, A.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Journal of Experimental Botany
Publisher:Oxford University Press
ISSN:0022-0957
ISSN (Online):1460-2431

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
419821Defence and defence suppression in plant pathogen interactions - the role of CaMV protein P6Joel MilnerBiotechnology and Biological Sciences Research Council (BBSRC)BB/D017319/1LS - BIOMOLECULAR SCIENCES