A quantitative study of neurochemically-defined excitatory interneuron populations in laminae I-III of the mouse spinal cord

Gutierrez-Mecinas, M. , Furuta, T., Watanabe, M. and Todd, A. J. (2016) A quantitative study of neurochemically-defined excitatory interneuron populations in laminae I-III of the mouse spinal cord. Molecular Pain, 12, pp. 1-18. (doi: 10.1177/1744806916629065) (PMID:27030714) (PMCID:PMC4946630)

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Abstract

Background: Excitatory interneurons account for the majority of neurons in laminae I–III, but their functions are poorly understood. Several neurochemical markers are largely restricted to excitatory interneuron populations, but we have limited knowledge about the size of these populations or their overlap. The present study was designed to investigate this issue by quantifying the neuronal populations that express somatostatin (SST), neurokinin B (NKB), neurotensin, gastrin-releasing peptide (GRP) and the γ isoform of protein kinase C (PKCγ), and assessing the extent to which they overlapped. Since it has been reported that calretinin- and SST-expressing cells have different functions, we also looked for co-localisation of calretinin and SST. Results: SST, preprotachykinin B (PPTB, the precursor of NKB), neurotensin, PKCγ or calretinin were detected with antibodies, while cells expressing GRP were identified in a mouse line (GRP-EGFP) in which enhanced green fluorescent protein (EGFP) was expressed under control of the GRP promoter. We found that SST-, neurotensin-, PPTB- and PKCγ-expressing cells accounted for 44%, 7%, 12% and 21% of the neurons in laminae I–II, and 16%, 8%, 4% and 14% of those in lamina III, respectively. GRP-EGFP cells made up 11% of the neuronal population in laminae I–II. The neurotensin, PPTB and GRP-EGFP populations showed very limited overlap, and we estimate that between them they account for ∼40% of the excitatory interneurons in laminae I–II. SST which is expressed by ∼60% of excitatory interneurons in this region, was found in each of these populations, as well as in cells that did not express any of the other peptides. Neurotensin and PPTB were often found in cells with PKCγ, and between them, constituted around 60% of the PKCγ cells. Surprisingly, we found extensive co-localisation of SST and calretinin. Conclusions: These results suggest that cells expressing neurotensin, NKB or GRP form largely non-overlapping sets that are likely to correspond to functional populations. In contrast, SST is widely expressed by excitatory interneurons that are likely to be functionally heterogeneous.

Item Type:Articles
Additional Information:The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: The work was supported by the Wellcome Trust (Grant Number 102645).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Gutierrez-Mecinas, Dr Maria and Todd, Professor Andrew
Authors: Gutierrez-Mecinas, M., Furuta, T., Watanabe, M., and Todd, A. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Neuroscience and Psychology
Journal Name:Molecular Pain
Publisher:Sage
ISSN:1744-8069
ISSN (Online):1744-8069
Published Online:08 December 2016
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Molecular Pain 12:1-18
Publisher Policy:Reproduced under a Creative Commons licence

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
644163Defining pain circuitry in health and diseaseAndrew ToddWellcome Trust (WELLCOME)102645/Z/13/ZINP - CENTRE FOR NEUROSCIENCE