Nitric oxide inhibits leucocyte migration in carrageenin-induced rat pleurisy

Ialenti, A., Ianaro, A., Maffia, P. , Sautebin, L. and Di Rosa, M. (2000) Nitric oxide inhibits leucocyte migration in carrageenin-induced rat pleurisy. Inflammation Research, 49(8), pp. 411-417. (doi: 10.1007/s000110050609) (PMID:11028758)

Abstract

Objective and Design: The role of nitric oxide (NO) on leucocyte migration has been investigated in rat carrageenin-induced pleurisy.Material: Male Wistar rats. Treatment: L-arginine, NOC-18 and aminoguanidine were administered subcutaneously 1 h prior to carrageenin injection. Methods: Leucocyte accumulation into the pleural cavity was measured 4 h after carrageenin challenge. Statistical significance was calculated by Bonferroni test. Results: L-arginine (10 mg/kg) or the NO donor NOC-18 (10 mg/kg), significantly inhibited leucocyte infiltration by 31% and 20% respectively (P <0.01). On the contrary, when these compounds were given at high doses (L-arginine 300 mg/kg; NOC-18 30 mg/kg), leucocyte accumulation was increased by 22% and 33% respectively (P <0.01). Aminoguanidine, a relatively selective inhibitor of the inducible NO synthase, depending on the dose, showed a biphasic effect on cell migration. Thus, at low doses (30 and 100 mg/kg), aminoguanidine increased (by 40% and 74% respectively, P <0.01) leucocyte infiltration which was inhibited by 41% (P <0.01) when the drug was given at high dose (300 mg/kg). Conclusions: These results suggest that in rat carrageenin-induced pleurisy NO primarily inhibits leucocyte migration.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Maffia, Professor Pasquale
• Authors: Ialenti, A., Ianaro, A., Maffia, P., Sautebin, L., and Di Rosa, M.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Inflammation Research
• Publisher: Birkhäuser Verlag
• ISSN: 1023-3830
• ISSN (Online): 1420-908X