Control of NF-κB subunits by ubiquitination

Collins, P. E., Colleran, A. and Carmody, R. J. (2015) Control of NF-κB subunits by ubiquitination. In: May, M.J. (ed.) NF-kappaB Methods and Protocols. Series: Methods in molecular biology (1280). Humana Press, pp. 355-370. ISBN 9781493924226 (doi: 10.1007/978-1-4939-2422-6_21)

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Abstract

NF-κB is an essential regulator of inflammation and is also required for normal immune development and homeostasis. The inducible activation of NF-κB by a wide range of immuno-receptors such as the toll-like receptors (TLR), Tumour Necrosis Factor receptor (TNFR), and antigen T cell and B cell receptors requires the ubiquitin-triggered proteasomal degradation of IκBα to promote the nuclear translocation and transcriptional activity of NF-κB dimers. More recently, an additional role for ubiquitination and proteasomal degradation in the control of NF-κB activity has been uncovered. In this case, it is the ubiquitination and proteasomal degradation of the NF-κB subunits that play a critical role in the termination of the NF-κB-dependent transcriptional response induced by receptor activation. The primary trigger of NF-κB ubiquitination is DNA binding by NF-κB dimers and is further controlled by specific phosphorylation events which regulate the interaction of NF-κB with the E3 ligase complex and the deubiquitinase enzyme USP7. It is the balance between ubiquitination and deubiquitination that shapes the NF-κB-mediated transcriptional response. This chapter describes methods for the analysis of NF-κB ubiquitination.

Item Type:Book Sections
Status:Published
Glasgow Author(s) Enlighten ID:Carmody, Dr Ruaidhri and Collins, Ms Patricia
Authors: Collins, P. E., Colleran, A., and Carmody, R. J.
Subjects:Q Science > Q Science (General)
Q Science > QH Natural history > QH345 Biochemistry
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Methods in molecular biology (Clifton, N.J.)
Publisher:Humana Press
ISSN:1940-6029
ISBN:9781493924226
Published Online:01 February 2015

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