Assessment of murine collagen-induced arthritis by longitudinal non-invasive duplexed molecular optical imaging

Scales, H. E. , Ierna, M., Smith, K. M., Ross, K., Meiklejohn, G. R., Patterson-Kane, J. C., McInnes, I. B., Brewer, J. M. , Garside, P. and Maffia, P. (2016) Assessment of murine collagen-induced arthritis by longitudinal non-invasive duplexed molecular optical imaging. Rheumatology, 55(3), pp. 564-572. (doi:10.1093/rheumatology/kev361) (PMID:26475798)

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Abstract

Objective. In the present study we evaluated the use of four commercially available fluorescent probes to monitor disease activity in murine CIA and its suppression during glucocorticoid therapy. Methods. Arthritis was induced in male DBA/1 mice by immunization with type II collagen in Complete Freund’s Adjuvant, followed by a boost of collagen in PBS. Four fluorescent probes from PerkinElmer in combination [ProSense 750 fluorescent activatable sensor technology (FAST) with Neutrophil Elastase 680 FAST and MMPSense 750 FAST with CatK 680 FAST] were used to monitor disease development from day 5 through to day 40 post-immunization. Fluorescence generated in vivo by the probes was correlated with clinical and histological score and paw measurements. Results. The fluorescence intensity emitted by each probe was shown to correlate with the conventional measurements of disease. The highest degree of correlation was observed with ProSense 750 FAST in combination with Neutrophil Elastase 680 FAST; these probes were then used to successfully assess CIA suppression during dexamethasone treatment. Conclusion. We have demonstrated that longitudinal non-invasive duplexed optical fluorescence imaging provides a simple assessment of arthritic disease activity within the joints of mice following the induction of CIA and may represent a powerful tool to monitor the efficacy of drug treatments in preclinical studies.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Maffia, Dr Pasquale and Garside, Professor Paul and Brewer, Professor James and Scales, Dr Hannah and Meiklejohn, Dr Gordon and Ross, Dr Kirsty
Authors: Scales, H. E., Ierna, M., Smith, K. M., Ross, K., Meiklejohn, G. R., Patterson-Kane, J. C., McInnes, I. B., Brewer, J. M., Garside, P., and Maffia, P.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Rheumatology
Publisher:Oxford University Press
ISSN:1462-0324
ISSN (Online):1462-0332
Published Online:16 October 2015
Copyright Holders:Copyright © 2015 The Authors
First Published:First published in Rheumatology 55(3):564-572
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
579801Transfer of expertise in bioimaging from academia to industry.Pasquale MaffiaBiotechnology and Biological Sciences Research Council (BBSRC)MB002 RBZ0174III -IMMUNOLOGY
579802Transfer of expertise in bioimaging from academia to industry.Pasquale MaffiaScottish Funding Council (SFC)MB002 RBZ0174III -IMMUNOLOGY

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