Metabolomic profiling of permethrin-treated Drosophila melanogaster identifies a role for tryptophan catabolism in insecticide survival

Brinzer, R., Henderson, L., Marchiondo, A. A., Woods, D. J., Davies, S. and Dow, J. A. T. (2015) Metabolomic profiling of permethrin-treated Drosophila melanogaster identifies a role for tryptophan catabolism in insecticide survival. Insect Biochemistry and Molecular Biology, 67, pp. 74-86. (doi:10.1016/j.ibmb.2015.09.009) (PMID:26474926)

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Abstract

Insecticides and associated synergists are rapidly losing efficacy in target insect pest populations making the discovery of alternatives a priority. To discover novel targets for permethrin synergists, metabolomics was performed on permethrin-treated Drosophila melanogaster. Changes were observed in several metabolic pathways including those for amino acids, glycogen, glycolysis, energy, nitrogen, NAD+, purine, pyrimidine, lipids and carnitine. Markers for acidosis, ammonia stress, oxidative stress and detoxification responses were also observed. Many of these changes had not been previously characterized after permethrin exposure. From the altered pathways, tryptophan catabolism was selected for further investigation. The knockdown of some tryptophan catabolism genes (vermilion, cinnabar and CG6950) in the whole fly and in specific tissues including fat body, midgut and Malpighian tubules using targeted RNAi resulted in altered survival phenotypes against acute topical permethrin exposure. The knockdown of vermilion, cinnabar and CG6950 in the whole fly also altered survival phenotypes against chronic oral permethrin, fenvalerate, DDT, chlorpyriphos and hydramethylnon exposure. Thus tryptophan catabolism has a previously uncharacterized role in defence against insecticides, and shows that metabolomics is a powerful tool for target identification in pesticide research.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Dow, Professor Julian and Henderson, Ms Louise and Davies, Professor Shireen
Authors: Brinzer, R., Henderson, L., Marchiondo, A. A., Woods, D. J., Davies, S., and Dow, J. A. T.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Insect Biochemistry and Molecular Biology
Publisher:Elsevier Ltd.
ISSN:0965-1748
ISSN (Online):1879-0240
Published Online:22 October 2015
Copyright Holders:Copyright © 2015 Elsevier Ltd.
First Published:First published in Insect Biochemistry and Molecular Biology 67: 74-86
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
609661FlyAtlas2: a definitive, expanded NGS expression resource for the Drosophila community and beyond.Julian DowBiotechnology and Biological Sciences Research Council (BBSRC)BB/K019953/1RI MOLECULAR CELL & SYSTEMS BIOLOGY