Domingues, P., Bamford, C. G.G., Boutell, C. and McLauchlan, J. (2015) Inhibition of hepatitis C virus RNA replication by ISG15 does not require its conjugation to protein substrates by the HERC5 E3 ligase. Journal of General Virology, 96(11), pp. 3236-3242. (doi: 10.1099/jgv.0.000283) (PMID:26361997) (PMCID:PMC4806579)
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Abstract
Chronic infection of the liver by hepatitis C virus (HCV) induces a range of host factors including IFN-stimulated genes such as ISG15. ISG15 functions as an antiviral factor that limits virus replication. Previous studies have suggested that ISG15 could influence HCV replication in both a positive and a negative manner. In this report, we determined the effect of ISG15 on HCV RNA replication in two independent cell lines that support viral genome synthesis by inhibiting ISG15 expression through small interfering RNA, short-hairpin RNA and CRISPR/Cas9 gene knockout approaches. Our results demonstrated that ISG15 impairs HCV RNA replication in both the presence and absence of IFN stimulation, consistent with an antiviral role for ISG15 during HCV infection. ISG15 conjugation to protein substrates typically requires the E3 ligase, HERC5. Our results showed that the inhibitory effect of ISG15 on HCV RNA replication does not require its conjugation to substrates by HERC5.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Domingues, Miss Patricia and Bamford, Dr Connor and Boutell, Dr Chris and McLauchlan, Professor John |
| Authors: | Domingues, P., Bamford, C. G.G., Boutell, C., and McLauchlan, J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Journal of General Virology |
| Publisher: | Microbiology Society |
| ISSN: | 0022-1317 |
| ISSN (Online): | 1465-2099 |
| Copyright Holders: | Copyright © 2015 The Authors |
| First Published: | First published in Journal of General Virology 96(11):3236-3243 |
| Publisher Policy: | Reproduced under a Creative Commons licence |
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