Stapleton, G. , Nguyen, C. P., Lease, K. A. and Hille, M. B. (1998) Phosphorylation of protein kinase C-related kinase PRK2 during meiotic maturation of starfish oocytes. Developmental Biology, 193(1), pp. 36-46. (doi: 10.1006/dbio.1997.8791) (PMID:9466886))
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Abstract
The resumption of meiosis in the developing starfish oocyte is the result of intracellular signaling events initiated by 1-methyladenine stimulation. One of the earliest detectable kinase activities during meiotic maturation of starfish oocytes is a protein kinase C or PKC-like activity. In this study, several isoforms of protein kinase C were cloned from the oocyte; however, the most abundant PKC-like maternal transcript corresponds to protein kinase C-related kinase 2 (PRK2). PRK2 is expressed in the immature oocyte and at least until germinal vesicle breakdown. Subcellular localization of PRK2 revealed a cytoplasmic distribution in the immature oocyte, which, during meiotic maturation, remained in the cytoplasm but also localized to the disintegrating germinal vesicle. Significantly, PRK2 is phosphorylatedin vivoin response to 1-methyladenine which precedes MPF activation, making PRK2 a candidate regulator of early signaling events of meiotic maturation.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Stapleton, Dr Genevieve |
Authors: | Stapleton, G., Nguyen, C. P., Lease, K. A., and Hille, M. B. |
College/School: | College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing |
Journal Name: | Developmental Biology |
Publisher: | Elsevier |
ISSN: | 0012-1606 |
ISSN (Online): | 1095-564X |
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