Phosphorylation of protein kinase C-related kinase PRK2 during meiotic maturation of starfish oocytes

Stapleton, G. , Nguyen, C. P., Lease, K. A. and Hille, M. B. (1998) Phosphorylation of protein kinase C-related kinase PRK2 during meiotic maturation of starfish oocytes. Developmental Biology, 193(1), pp. 36-46. (doi: 10.1006/dbio.1997.8791) (PMID:9466886))

Full text not currently available from Enlighten.

Abstract

The resumption of meiosis in the developing starfish oocyte is the result of intracellular signaling events initiated by 1-methyladenine stimulation. One of the earliest detectable kinase activities during meiotic maturation of starfish oocytes is a protein kinase C or PKC-like activity. In this study, several isoforms of protein kinase C were cloned from the oocyte; however, the most abundant PKC-like maternal transcript corresponds to protein kinase C-related kinase 2 (PRK2). PRK2 is expressed in the immature oocyte and at least until germinal vesicle breakdown. Subcellular localization of PRK2 revealed a cytoplasmic distribution in the immature oocyte, which, during meiotic maturation, remained in the cytoplasm but also localized to the disintegrating germinal vesicle. Significantly, PRK2 is phosphorylatedin vivoin response to 1-methyladenine which precedes MPF activation, making PRK2 a candidate regulator of early signaling events of meiotic maturation.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Stapleton, Dr Genevieve
Authors: Stapleton, G., Nguyen, C. P., Lease, K. A., and Hille, M. B.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
Journal Name:Developmental Biology
Publisher:Elsevier
ISSN:0012-1606
ISSN (Online):1095-564X

University Staff: Request a correction | Enlighten Editors: Update this record