Characterizing the trigger for sarcoplasmic reticulum Ca2+ release in cardiac myocytes

Bito, V., MacQuaide, N. and Sipido, K. R. (2015) Characterizing the trigger for sarcoplasmic reticulum Ca2+ release in cardiac myocytes. Cold Spring Harbor Protocols, 2015(4), pp. 398-402. (doi: 10.1101/pdb.prot076968) (PMID:25834258)

Abstract

Here, we describe a method for characterizing the L-type Ca(2+) current, ICaL, which is a major trigger for Ca(2+) release from the sarcoplasmic reticulum (SR). The protocol includes measuring ICaL amplitude and voltage dependence and the elicited SR Ca(2+) release. The procedure for measuring ICaL activity is performed using solutions (internal and external) and voltage control such that other ionic currents are eliminated. The resultant relationship between the Ca(2+) current and the associated internal [Ca(2+)]i transient is a first approach for evaluating coupling gain. We discuss which parameters are most appropriate for this analysis and how an evaluation of gain needs to be further explored by measuring the SR Ca(2+) content.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: MacQuaide, Dr Niall
• Authors: Bito, V., MacQuaide, N., and Sipido, K. R.
• College/School: College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
• Journal Name: Cold Spring Harbor Protocols
• Publisher: Cold Spring Harbor Laboratory Press
• ISSN: 1940-3402
• ISSN (Online): 1559-6095