A systematic approach for assessing Ca²⁺ handling in cardiac myocytes

Sipido, K. R., MacQuaide, N. and Bito, V. (2015) A systematic approach for assessing Ca²⁺ handling in cardiac myocytes. Cold Spring Harbor Protocols, 2015(5), pp. 431-433. (doi: 10.1101/pdb.top066142) (PMID:25934941)

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Abstract

In cardiac myocytes, Ca(2+) release from the sarcoplasmic reticulum (SR) Ca(2+) store through the opening of ryanodine receptors (RyRs) is the major source of Ca(2+) for activation of myofilaments and contraction. Over the past 20 years, tools have become available to study this release process in detail, allowing new insights into the regulation of SR Ca(2+) release and RyR function. To assess these processes, we recommend and here review a systematic approach that evaluates the essential transport mechanisms and Ca(2+) fluxes in isolated single cardiac myocytes by using fluorescent Ca(2+) indicators and whole-cell recording of membrane voltage and ionic currents under voltage clamp. The approach includes an assessment of the L-type Ca(2+) current as a trigger for opening of RyRs and release of SR Ca(2+), of the SR Ca(2+) content, of intrinsic properties of RyRs, and of Ca(2+)-removal systems.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:MacQuaide, Dr Niall
Authors: Sipido, K. R., MacQuaide, N., and Bito, V.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:Cold Spring Harbor Protocols
Publisher:Cold Spring Harbor Laboratory Press
ISSN:1940-3402
ISSN (Online):1559-6095

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