Alles, J. et al. (2015) Epstein-Barr virus EBER transcripts affect miRNA-mediated regulation of specific targets and are processed to small RNA species. Non-Coding RNA, 1(3), pp. 170-191. (doi: 10.3390/ncrna1030170)
|
Text
114202.pdf - Published Version Available under License Creative Commons Attribution. 673kB |
Abstract
The oncogenic Epstein-Barr virus (EBV) expresses 44 mature microRNAs and two non-coding EBER RNAs of 167 (EBER1) and 172 (EBER2) nt length. MiRNA profiling of NK/T cell lines and primary cells and Northern blotting of EBV-infected cell lines and primary tumors revealed processing of EBER1 to short 5′-derived RNAs of approximately 23, 52 and 70 nt (EBER123, EBER152, and EBER170) and of EBER2 to 3′ fragments. The biogenesis of these species is independent of Dicer, and EBER123 does not act like a miRNA OPEN ACCESS Non-Coding RNA 2015, 1 171 to target its complementary sequence. EBER1, EBER2 and EBER123 were bound by the lupus antigen (La), a nuclear and cytoplasmic protein that facilitates RNAi. Consistent with this, the EBERs affect regulation of interleukin 1alpha (IL1α) and RAC1 reporters harboring miR target sequences, targets of miR-142-3p. However, the EBERs have no effect upon another target of miR-142-3p, ADCY9, nor on TOMM22, a target of ebv-miR-BART16, indicative of selective modulation of gene expression by the EBERs.
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Wilson, Professor Joanna and Tesson, Dr Mathias and Hamilton, Dr Andrew |
Authors: | Alles, J., Hasler, D., Kazmi, S. M. A., Tesson, M., Hamilton, A., Schlegel, L., Marx, S., Eichner, N., Reinhardt, R., Meister, G., Wilson, J. B., and Grässer, F. A. |
Subjects: | Q Science > QR Microbiology > QR355 Virology |
College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
Journal Name: | Non-Coding RNA |
Publisher: | M D P I AG |
ISSN: | 2311-553X |
ISSN (Online): | 2311-553X |
Copyright Holders: | Copyright © 2015 The Authors |
First Published: | First published in Non-Coding RNA 1(3):170-191 |
Publisher Policy: | Reproduced under a Creative Commons License |
University Staff: Request a correction | Enlighten Editors: Update this record