Epstein-Barr virus EBER transcripts affect miRNA-mediated regulation of specific targets and are processed to small RNA species

Alles, J. et al. (2015) Epstein-Barr virus EBER transcripts affect miRNA-mediated regulation of specific targets and are processed to small RNA species. Non-Coding RNA, 1(3), pp. 170-191. (doi: 10.3390/ncrna1030170)

[img]
Preview
Text
114202.pdf - Published Version
Available under License Creative Commons Attribution.

673kB

Abstract

The oncogenic Epstein-Barr virus (EBV) expresses 44 mature microRNAs and two non-coding EBER RNAs of 167 (EBER1) and 172 (EBER2) nt length. MiRNA profiling of NK/T cell lines and primary cells and Northern blotting of EBV-infected cell lines and primary tumors revealed processing of EBER1 to short 5′-derived RNAs of approximately 23, 52 and 70 nt (EBER123, EBER152, and EBER170) and of EBER2 to 3′ fragments. The biogenesis of these species is independent of Dicer, and EBER123 does not act like a miRNA OPEN ACCESS Non-Coding RNA 2015, 1 171 to target its complementary sequence. EBER1, EBER2 and EBER123 were bound by the lupus antigen (La), a nuclear and cytoplasmic protein that facilitates RNAi. Consistent with this, the EBERs affect regulation of interleukin 1alpha (IL1α) and RAC1 reporters harboring miR target sequences, targets of miR-142-3p. However, the EBERs have no effect upon another target of miR-142-3p, ADCY9, nor on TOMM22, a target of ebv-miR-BART16, indicative of selective modulation of gene expression by the EBERs.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Wilson, Professor Joanna and Tesson, Dr Mathias and Hamilton, Dr Andrew
Authors: Alles, J., Hasler, D., Kazmi, S. M. A., Tesson, M., Hamilton, A., Schlegel, L., Marx, S., Eichner, N., Reinhardt, R., Meister, G., Wilson, J. B., and Grässer, F. A.
Subjects:Q Science > QR Microbiology > QR355 Virology
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
Journal Name:Non-Coding RNA
Publisher:M D P I AG
ISSN:2311-553X
ISSN (Online):2311-553X
Copyright Holders:Copyright © 2015 The Authors
First Published:First published in Non-Coding RNA 1(3):170-191
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record